Prostate cancer is a heterogeneous disease and considered to be the most commonly diagnosed cancer. SFRP4 gene acts as Wnt antagonist in the Wnt signalling pathway, thereby playing an important role in carcinogenesis. The aim of the present study was to investigate two single-nucleotide polymorphisms: c.958 C>A (rs1802073) and c.1019 G>A (rs1802074) in the SFRP4 gene and its expression in prostate cancer. A sample size of 100 cases and 100 age-matched controls were recruited for the study. Statistical analysis revealed the heterozygous GA genotype of rs1802074 significantly increased in cases when compared to controls. Analysis of sFRP4 expression based on the genotypes showed a significantly increased expression for the heterozygous GA and homozygous AA genotypes in cases when compared to the controls. Fold change was calculated using 2 −ΔΔ CT method and the results showed that there were a 3.4 and 4.5 fold increase in the sFRP4 expression for GA and AA genotypes, respectively. Our results suggest that the rs1802074 polymorphism in SFRP4 gene may be associated with the risk of prostate cancer.

中文翻译：

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SFRP4基因多态性及在前列腺癌中的表达研究

前列腺癌是一种异质性疾病，被认为是最常见的癌症。SFRP4基因在Wnt信号通路中作为Wnt拮抗剂发挥作用，在致癌作用中发挥重要作用。本研究的目的是调查 SFRP4 基因中的两个单核苷酸多态性：c.958 C>A (rs1802073) 和 c.1019 G>A (rs1802074) 及其在前列腺癌中的表达。本研究招募了 100 名病例和 100 名年龄匹配的对照样本。统计分析显示，与对照相比，rs1802074 的杂合 GA 基因型在病例中显着增加。基于基因型的 sFRP4 表达分析显示，与对照相比，杂合 GA 和纯合 AA 基因型的表达显着增加。使用 2 -ΔΔ CT 方法计算倍数变化，结果显示 GA 和 AA 基因型的 sFRP4 表达分别增加了 3.4 和 4.5 倍。我们的研究结果表明，SFRP4 基因中的 rs1802074 多态性可能与前列腺癌的风险有关。