The present study was an investigation of the neuroprotective effects of probiotic bacteria in SH-SY5Y neuroblastoma cells experiencing oxidative stress. The bacterial strains were: commercial Lactobacillus rhamnosus GG; two isolated bacterial strains (Lactobacillus delbrueckii KU200170 and Lactobacillus plantarum KU200661); and probiotic Lactococcus lactis KC24. To evaluate the neuroprotective effects of the bacteria, a conditioned medium (CM) was prepared using HT-29 cells cultured with the heat-killed probiotic strains. Of the bacterial strains tested, the oxidatively stressed SH-SY5Y cells were most viable when cultured with L. lactis KC24-CM. L. lactis KC24-CM promoted the expression of brain-derived neurotropic factor (BDNF) in the HT-29 cells. It also significantly increased BDNF expression and reduced the apoptosis-related Bax/Bcl-2 ratio in the oxidatively stressed SH-SY5Y cells. Therefore, L. lactis KC24 is a potential psychobiotic for use in the functional food industry.

本研究调查了益生菌对经历氧化应激的 SH-SY5Y 神经母细胞瘤细胞的神经保护作用。菌株为：商品鼠李糖乳杆菌GG；两个分离的细菌菌株（德氏乳杆菌KU200170 和植物乳杆菌KU200661）；和益生菌乳酸乳球菌KC24。为了评估细菌的神经保护作用，使用与热灭活的益生菌菌株一起培养的 HT-29 细胞制备条件培养基 (CM)。在测试的细菌菌株中，当与乳酸乳球菌KC24-CM一起培养时，氧化应激的 SH-SY5Y 细胞最有活力。乳酸乳球菌KC24-CM促进HT-29细胞中脑源性神经营养因子( BDNF )的表达。它还显着增加了BDNF表达并降低了氧化应激 SH-SY5Y 细胞中与细胞凋亡相关的Bax/Bcl-2比率。因此，乳酸乳球菌KC24 是一种用于功能性食品工业的潜在精神生物。