Advances in CRISPR technology have immensely improved our ability to manipulate nucleic acids, and the recent discovery of the RNA-targeting endonuclease Cas13 adds even further functionality. Here, we show that Cas13 works efficiently in Drosophila , both ex vivo and in vivo. We test 44 different Cas13 variants to identify enzymes with the best overall performance and show that Cas13 could target endogenous Drosophila transcripts in vivo with high efficiency and specificity. We also develop Cas13 applications to edit mRNAs and target mitochondrial transcripts. Our vector collection represents a versatile tool collection to manipulate gene expression at the post-transcriptional level.

中文翻译：

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用于果蝇中基于 CRISPR-Cas13 的 RNA 操作的多功能工具包


CRISPR 技术的进步极大地提高了我们操作核酸的能力，而最近发现的 RNA 靶向核酸内切酶 Cas13 甚至增加了更多功能。在这里，我们证明 Cas13 在果蝇体内和离体体内都能有效发挥作用。我们测试了 44 种不同的 Cas13 变体，以确定具有最佳整体性能的酶，并表明 Cas13 可以在体内高效、特异性地靶向内源果蝇转录本。我们还开发 Cas13 应用程序来编辑 mRNA 和靶向线粒体转录本。我们的载体集合代表了在转录后水平操纵基因表达的多功能工具集合。