The eukaryotic genome is hierarchically structured yet highly dynamic. Regulating transcription in this environment demands a high level of coordination to permit many proteins to interact with chromatin fiber at appropriate sites in a timely manner. We describe how recent advances in quantitative imaging techniques overcome caveats of sequencing-based methods (Hi-C and related) by enabling direct visualization of transcription factors and chromatin at high resolution, from single genes to the whole nucleus. We discuss the contribution of fluorescence imaging to deciphering the principles underlying this coordination within the crowded nuclear space in living cells and discuss challenges ahead.

中文翻译：

---

引导人群：可视化基因组动力学、结构和转录之间的协调


真核基因组具有层次结构，但高度动态。在这种环境中调节转录需要高水平的协调，以允许许多蛋白质及时地在适当的位点与染色质纤维相互作用。我们描述了定量成像技术的最新进展如何通过以高分辨率直接可视化从单个基因到整个细胞核的转录因子和染色质，从而克服基于测序的方法（Hi-C 和相关）的注意事项。我们讨论了荧光成像对破译活细胞拥挤的核空间内这种协调的原理的贡献，并讨论了未来的挑战。