Objective: The present study was conducted to elucidate the protective effect of Casticin against chronic obstructive pulmonary disease (COPD) in rats.
Methods: The COPD in rats was induced by the controlled cigarette smoke, and CST (10, 20, and 30 mg/kg) was injected into the cigarette-smoke exposed rats. Blood was taken from the abdominal vein and centrifuged (1500×g, 4°C, 15min); plasma was collected and used for the determination of various biochemical parameters.
Results: The results of the study suggested that CST significantly improved the lung functions of the rats in a dose-dependent manner. It also causes a reduction of white blood cells, neutrophils, and macrophages in BALF of rats. The plasma level of leptin and C-reactive protein together with pro-inflammatory cytokines (TNF-α, IL-1β, and IL-6) were also significantly restored to near to normal in CST-treated group. In Western blot analysis, CST causes significant inhibition of the NF-ĸB and iNOS pathway.
Conclusion: Our study demonstrated that the CST protects lungs against COPD via improving lung functions and inhibition of oxidative stress and inflammation.

Keywords: COPD, Casticin, smoke, inflammation, oxidative stress, NF-ĸB


中文翻译：

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Casticin 通过抑制 NF-ĸB 在慢性香烟烟雾暴露大鼠慢性阻塞性肺疾病模型中改善呼吸功能障碍并减轻氧化应激和炎症

目的：本研究旨在阐明Casticin对大鼠慢性阻塞性肺病（COPD）的保护作用。
方法：通过控制香烟烟雾诱导大鼠COPD，将CST（10、20和30 mg/kg）注射到暴露于香烟烟雾的大鼠体内。从腹静脉取血并离心（1500×g，4°C，15min）；收集血浆并用于测定各种生化参数。
结果：研究结果表明，CST 以剂量依赖性方式显着改善了大鼠的肺功能。它还导致大鼠 BALF 中的白细胞、中性粒细胞和巨噬细胞减少。在 CST 治疗组中，瘦素和 C 反应蛋白以及促炎细胞因子（TNF-α、IL-1β 和 IL-6）的血浆水平也显着恢复至接近正常水平。在蛋白质印迹分析中，CST 显着抑制 NF-ĸB 和 iNOS 通路。
结论：我们的研究表明，CST 通过改善肺功能和抑制氧化应激和炎症来保护肺免受 COPD。

关键词： COPD，Casticin，烟雾，炎症，氧化应激，NF-ĸB