MicroRNA expression levels highly correlate with the occurrence, diagnosis and prognosis of disease. However, challenges remain in establishing a multiplex and fast detection method. Here, we developed a multiplex and fast detection platform for microRNAs based on a self-priming microfluidic chip and duplex-specific nuclease. It can detect three types of miRNAs, including miR-100, miR-155, and Let-7a, simultaneously at 50 °C within 1 h. The probes are pre-introduced into the chip using the self-priming method and cross-contamination can be avoided by using a screw valve. The reagent consumption and cost have been largely reduced in this work compared to the traditional detection method. This chip also exhibits good quantitative performance and specificity. As a proof of concept, we detect three types of microRNAs from different cancer cell lines, which demonstrates its potential in real sample analysis. In summary, this microfluidic chip shows great advantages in multiplex, fast and simple detection of microRNAs, and possesses great potential in the early diagnosis of miRNA-related diseases, especially for point-of-care application.

中文翻译：

---

基于自吸微流控芯片和双链体特异性核酸酶的microRNA多重检测平台

MicroRNA表达水平与疾病的发生，诊断和预后高度相关。然而，建立多重和快速检测方法仍然面临挑战。在这里，我们基于自吸微流控芯片和双链特异性核酸酶开发了一种用于microRNA的多重快速检测平台。它可以在50°C下在1小时内同时检测到三种类型的miRNA，包括miR-100，miR-155和Let-7a。使用自吸方法将探针预先引入芯片中，并且可以使用螺旋阀避免交叉污染。与传统的检测方法相比，这项工作大大减少了试剂的消耗和成本。该芯片还具有良好的定量性能和特异性。作为概念证明，我们从不同的癌细胞系中检测出三种类型的microRNA，证明了其在实际样品分析中的潜力。综上所述，这种微流控芯片在微RNA的多重，快速和简单检测中显示出巨大的优势，并且在与miRNA相关的疾病的早期诊断中具有巨大潜力，尤其是在即时医疗应用中。