Neuraminidase plays an essential role in the spread of influenza viruses via cleaving sialic acids from the host cell receptors and virions. Neuraminidase has been regarded as an essential target for prevention and treatment of influenza infection. The one-step high-performance liquid chromatography-fraction collector (HPLC-FC) was selected to prepare fractions from Reduning (RDN) injection, while ultra-high-performance liquid chromatography/quadrupole-time-of-flight mass spectrometry (UHPLC/Q-TOF-MS) was used to identify fractions depending on their retention time and molecular ion. As a result, 75 fractions were prepared and 28 fractions out of them exhibited NA inhibitory effects with the dose-effect relationship. Exploring it further, six components including neochlorogenic acid, chlorogenic acid, cryptochlorogenic acid, isochlorogenic acid B, isochlorogenic acid A, and isochlorogenic acid C were the main components that accounted for almost 80% of inhibitory activity of RDN injection. Accordingly, these results demonstrated that this strategy could not only rapidly identify but also accurately screen active components from traditional Chinese medicine.

中文翻译：

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通过一步高效液相色谱-馏分收集器和UHPLC / Q-TOF-MS鉴定并从注射液中回收天然神经氨酸酶抑制剂

神经氨酸酶通过从宿主细胞受体和病毒体中裂解唾液酸，在流感病毒的传播中起重要作用。神经氨酸酶已被认为是预防和治疗流感感染的重要靶标。选择一步高效液相色谱-馏分收集器（HPLC-FC）从Reduning（RDN）进样中制备馏分，而超高效液相色谱/四极杆飞行时间质谱（UHPLC / Q-TOF-MS）用于根据馏分的保留时间和分子离子鉴定馏分。结果，制备了75个级分，其中28个级分显示出具有剂量-效应关系的NA抑制作用。进一步探讨包括新绿原酸，绿原酸，隐绿原酸，异绿原酸B在内的六个成分，异绿原酸A和异绿原酸C是占RDN注射抑制活性近80％的主要成分。因此，这些结果表明，该策略不仅可以快速识别，而且可以准确地从中药中筛选出有效成分。