Fourier ptychographic microscopy (FPM) is a recently developed computational imaging technique that has high-resolution and wide field-of-view (FOV). FPM bypasses the NA limit of the system by stitching a number of variable-illuminated measured images in Fourier space. On the basis of the wide FOV of the low NA objective, the high-resolution image with a wide FOV can be reconstructed through the phase recovery algorithm. However, the high-resolution reconstruction images are affected by the LED array point light source. The results are: (1) the intensities collected by the sample are severely declined when edge LEDs illuminate the sample; (2) the multiple reconstructions are caused by wavevectors inconsistency for the full FOV images. Here, we propose a new lighting scheme termed full FOV Fourier ptychographic microscopy (F³PM). By combining the LED array and telecentric lens, the method can provide plane waves with different angles while maintaining uniform intensity. Benefiting from the telecentric performance and f‒θ property of the telecentric lens, the system stability is improved and the relationship between the position of LED and its illumination angle is simplified. The excellent plane wave provided by the telecentric lens guarantees the same wavevector in the full FOV, and we use this wavevector to reconstruct the full FOV during one time. The area and diameter of the single reconstruction FOV reached 14.6mm² and 5.4 mm, respectively, and the diameter is very close to the field number (5.5 mm) of the 4× objective. Compared with the traditional FPM, we have increased the diameter of FOV in a single reconstruction by ∼ 10 times, eliminating the complicated steps of computational redundancy and image stitching.

中文翻译：

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单个全FOV重建傅里叶色谱分析

傅里叶气相色谱（FPM）是一种最新开发的计算成像技术，具有高分辨率和宽视场（FOV）。FPM通过在傅立叶空间中拼接许多可变照明的测量图像来绕过系统的NA限制。在低NA物镜的宽视场的基础上，可以通过相位恢复算法重建宽视场的高分辨率图像。然而，高分辨率重建图像受到LED阵列点光源的影响。结果是：（1）当边缘LED照亮样品时，样品收集的强度严重下降；（2）多次重构是由完整FOV图像的波矢不一致引起的。在这里，我们提出了一种新的照明方案，称为全FOV傅立叶色谱分析（F下午³点）。通过组合LED阵列和远心透镜，该方法可以提供具有不同角度的平面波，同时保持均匀的强度。从远心性能和受益˚F - θ远心透镜的特性，系统的稳定性得到提高，LED的位置和其照射角之间的关系被简化。远心镜头提供的出色平面波可确保整个FOV中的波矢量相同，因此我们可以使用此波矢量在一次时间内重建整个FOV。单个重建视场的面积和直径分别达到14.6 mm ²和5.4 mm，并且直径非常接近视场数（5.5 mm）的4倍物镜。与传统的FPM相比，我们在一次重构中将FOV的直径增加了约10倍，从而消除了计算冗余和图像拼接的复杂步骤。