Abstract

Purpose

To evaluate whether the additive needle tract ablation (TA) can reduce adherent cells on the needle tract after radiofrequency ablation (RFA) in a preclinical HCC mouse model.

Methods

Hep3B-Luc cells were engrafted in the Balb/c-nude mice. Nineteen mice were randomly assigned into three groups: the needle only group (needle placement only without performing RFA), the RFA only group (needle placement with active RFA treatment), and the RFA-TA group (needle placement with active RFA treatment and additive tract ablation). The 17-gauge needle with a 10-mm active tip was used. After RFA and TA, the viability of adherent tumor cells on the RFA needle was evaluated with bioluminescence imaging (BLI) and live-cell counting.

Results

We observed that RFA-TA group had the lowest BLI values compared with other groups (needle only group, 11.2 ± 6.4 million; RFA only group, 13.6 ± 9.1 million; RFA-TA group, 1.11 ± 0.8 million, p = 0.001). Live cell counting with acridine orange/propidium iodide staining also confirmed that the counted viable cell numbers in RFA-TA group were lowest compared to the other groups (needle only group, 14.8 ± 4.5; RFA only group, 643.8 ± 131.9; RFA-TA group, 1.5 ± 0.9, p < 0.001).

Conclusions

The additive tract ablation can significantly reduce the number of viable tumor cells adherent to the RFA needle, which can prevent needle tract seeding after RFA procedure.

中文翻译：

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射频消融后进行导管消融，以防止肿瘤细胞粘附到针状电极上

摘要

目的

为了评估在临床前HCC小鼠模型中射频消融（RFA）后，附加针道消融（TA）是否可以减少针道上的粘附细胞。

方法

Hep3B-Luc细胞被植入Balb / c-裸鼠中。将19只小鼠随机分为三组：仅针组（仅在不进行RFA的情况下仅放置针头），仅RFA组（经有效RFA治疗的针头放置）和RFA-TA组（经有效RFA处理和添加物的针头放置）导管消融）。使用具有10毫米有源针尖的17号针头。在RFA和TA后，通过生物发光成像（BLI）和活细胞计数评估RFA针上粘附的肿瘤细胞的生存能力。

结果

我们观察到，与其他组相比，RFA-TA组的BLI值最低（仅针组为11.2±640万；仅RFA组为13.6±910万； RFA-TA组为1.11±80万，p  = 0.001）。cr啶橙/碘化丙锭染色的活细胞计数也证实，与其他组相比，RFA-TA组的活细胞计数最低（仅针组，14.8±4.5；仅RFA组，643.8±131.9； RFA-TA组，1.5±0.9，p  <0.001）。

结论

附加道消融可以显着减少附着在RFA针上的活瘤细胞的数量，从而可以防止RFA手术后接种针道。