Abstract

Hair waste from unhairing of bovine hide has high protein content and is also a current issue in tanneries as it causes high organic load to the wastewater. Given that, this study aims to recover keratin from bovine hair by investigating its hydrolysis from remaining solid hair from leather processing and unprocessed hair. For the keratin hydrolysis, 2-mercaptoethanol, Na₂S, H₂O₂, NaOCl, NaOH, C₂H₄O₂S, and HCOOH were tested. SDS-PAGE and the yield analysis of the extracted solution indicated that it was possible to recover proteins with molecular weights around 20 kDa, reusing this hair waste. In an oxidizing pathway (H₂O₂), 75% of the protein was extracted from the recovered hair powder, and a yield of up to 40% was achieved with Na₂S in a reductive pathway. Overall, the results demonstrated good recovery of keratin from bovine hair, turning what was previously considered waste into an interesting source of this protein.

摘要

牛皮脱毛产生的毛发废料具有高蛋白质含量，也是制革厂当前的一个问题，因为它会给废水带来高有机负荷。鉴于此，本研究旨在通过研究皮革加工和未加工毛发中剩余的固体毛发的水解作用，从牛毛中回收角蛋白。对于角蛋白水解，测试了2-巯基乙醇、Na ₂ S、H ₂ O ₂、NaOCl、NaOH、C ₂ H ₄ O ₂ S和HCOOH。提取溶液的 SDS-PAGE 和产量分析表明，可以回收分子量约为 20 kDa 的蛋白质，再利用这些头发废料。在氧化途径中（H ₂ O ₂)，从回收的发粉中提取出75%的蛋白质，并且在还原途径中使用Na _{2 S实现了高达40%的收率。}总体而言，结果表明从牛毛中很好地回收了角蛋白，将以前被认为是废物的东西变成了这种蛋白质的有趣来源。