Foot‐and‐mouth disease (FMD) is a major viral disease in farm animals. In the present study, seven monoclonal antibodies (mAbs) were produced against the FMD virus (FMDV)‐encoded RNA‐dependent RNA polymerase (3D protein) and characterized. Screening of mAb reactivity against three overlapping fragments of the 3D protein expressed in Escherichia coli revealed that the binding sites of all the mAbs were confined to the N‐terminal one‐third of the 3D protein. A selected mAb was utilized for detecting FMDV in the infected cell culture and tissues obtained from FMDV‐infected animals.

中文翻译：

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用于检测病毒感染的针对口蹄疫病毒 RNA 聚合酶的单克隆抗体

口蹄疫（FMD）是农场动物的主要病毒性疾病。在本研究中，针对口蹄疫病毒 (FMDV) 编码的 RNA 依赖性 RNA 聚合酶（3D 蛋白）产生了七种单克隆抗体 (mAb) 并对其进行了表征。对在大肠杆菌中表达的 3D 蛋白的三个重叠片段的 mAb 反应性的筛选表明，所有 mAb 的结合位点仅限于 3D 蛋白的 N 端三分之一。选定的 mAb 用于检测感染细胞培养物和从 FMDV 感染动物获得的组织中的 FMDV。