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Irreversible Adsorption of Serum Proteins onto Nanoparticles
Particle & Particle Systems Characterization ( IF 2.7 ) Pub Date : 2020-11-17 , DOI: 10.1002/ppsc.202000273 Cornelia Baeckmann 1 , Hanspeter Kählig 2 , Mika Lindén 3 , Freddy Kleitz 1
Particle & Particle Systems Characterization ( IF 2.7 ) Pub Date : 2020-11-17 , DOI: 10.1002/ppsc.202000273 Cornelia Baeckmann 1 , Hanspeter Kählig 2 , Mika Lindén 3 , Freddy Kleitz 1
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When a material comes in contact with serum or plasma, proteins will immediately adsorb to its surface. The extent of serum protein adsorption as well as the composition of the protein corona is thought to be decisive for the biological fate. The understanding of the mechanism underlying the concurrent adsorption of multiple proteins and the exact ways by which the adsorbed proteins interact with the biological setting, is still rudimentary. For both cases, a correct estimate of the composition of the protein corona is the key for an improved understanding. The protein corona composition is typically analyzed indirectly through analysis of the supernatant after protein desorption. However, in most cases the particles are not analyzed afterward in order to ensure that all proteins indeed have desorbed. Here, the results related to the analysis of the amounts of proteins in the corona are reported, focusing on the desorbed as well as the fraction of proteins that do not desorb. Irreversible protein adsorption can be observed in some cases. The results show that, in addition of the analysis of the supernatant, analysis of the particles is of critical importance to fully characterize the protein corona formed on nanoparticles.
中文翻译：
血清蛋白在纳米颗粒上的不可逆吸附
当某种物质与血清或血浆接触时，蛋白质将立即吸附到其表面。血清蛋白吸附的程度以及蛋白电晕的组成被认为对生物命运起决定性作用。对多种蛋白质同时吸附的潜在机制以及被吸附蛋白质与生物学环境相互作用的确切方式的理解仍然是基本的。对于这两种情况，正确估计蛋白电晕的组成是增进了解的关键。通常通过对蛋白质脱附后的上清液的分析来间接分析蛋白质电晕的组成。然而，在大多数情况下，为了确保所有蛋白质确实已解吸，随后不对颗粒进行分析。这里，报告了与电晕中蛋白质含量分析有关的结果，重点是解吸的蛋白质以及未解吸的蛋白质的比例。在某些情况下，可以观察到不可逆的蛋白质吸附。结果表明，除了对上清液进行分析外，对颗粒的分析对于全面表征在纳米颗粒上形成的蛋白质电晕至关重要。
更新日期：2021-01-19
中文翻译：
血清蛋白在纳米颗粒上的不可逆吸附
当某种物质与血清或血浆接触时，蛋白质将立即吸附到其表面。血清蛋白吸附的程度以及蛋白电晕的组成被认为对生物命运起决定性作用。对多种蛋白质同时吸附的潜在机制以及被吸附蛋白质与生物学环境相互作用的确切方式的理解仍然是基本的。对于这两种情况，正确估计蛋白电晕的组成是增进了解的关键。通常通过对蛋白质脱附后的上清液的分析来间接分析蛋白质电晕的组成。然而，在大多数情况下，为了确保所有蛋白质确实已解吸，随后不对颗粒进行分析。这里，报告了与电晕中蛋白质含量分析有关的结果，重点是解吸的蛋白质以及未解吸的蛋白质的比例。在某些情况下，可以观察到不可逆的蛋白质吸附。结果表明，除了对上清液进行分析外，对颗粒的分析对于全面表征在纳米颗粒上形成的蛋白质电晕至关重要。
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