Investigation of intestinal transportation of peptide‐displaying bacteriophage particles using phage display method,Journal of Peptide Science

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Investigation of intestinal transportation of peptide‐displaying bacteriophage particles using phage display method
Journal of Peptide Science ( IF 1.8 ) Pub Date : 2020-11-16 , DOI: 10.1002/psc.3292
Hakimeh Kanaani _{1,

2} , Yadollah Azarmi ₂ , Siavoush Dastmalchi _{1,

2} , Omid Zarei ₃ , Maryam Hamzeh-Mivehroud _{1,

2}

Affiliation

To investigate whether peptide sequences with specific translocation across the gastrointestinal barrier can be identified as drug delivery vehicles, in vivo phage display was conducted. For this purpose, a random library of 12‐mer peptides displayed on M13 bacteriophage was orally administered to mice followed by recovery of the phage particles from the blood samples after three consecutive biopanning rounds. The obtained peptide sequences were analyzed using bioinformatics tools and software. The results demonstrated that M13 bacteriophage bearing peptides translocate nonspecifically across the mice intestinal mucosal barrier deduced from random distribution of amino acids in different positions of the identified peptide sequences. The most probable reason for entering the phage particles into systemic circulation after oral administration of the peptide library can be related to the nanoscale nature of their structures which provides a satisfying platform for the purpose of designing nanocarriers in pharmaceutical applications.

中文翻译：

噬菌体展示法研究肽展示噬菌体颗粒的肠道转运

为了研究具有跨胃肠屏障的特异性易位的肽序列是否可以被鉴定为药物递送载体，进行了体内噬菌体展示。为此，将 M13 噬菌体上展示的 12 聚体肽随机文库口服给小鼠，然后在连续三轮生物淘选后从血样中回收噬菌体颗粒。使用生物信息学工具和软件分析获得的肽序列。结果表明，携带肽的 M13 噬菌体非特异性地跨小鼠肠粘膜屏障易位，这是从已鉴定肽序列不同位置的氨基酸随机分布推断出来的。

更新日期：2020-11-16

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