Hybrid Printing Using Cellulose Nanocrystals Reinforced GelMA/HAMA Hydrogels for Improved Structural Integration,Advanced Healthcare Materials

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Hybrid Printing Using Cellulose Nanocrystals Reinforced GelMA/HAMA Hydrogels for Improved Structural Integration
Advanced Healthcare Materials ( IF 10.0 ) Pub Date : 2020-11-16 , DOI: 10.1002/adhm.202001410
Yuchao Fan ₁ , Zhilian Yue ₁ , Enrico Lucarelli ₂ , Gordon G. Wallace ₁

Affiliation

3D printing of soft‐tissue like cytocompatible single material constructs with appropriate mechanical properties remains a challenge. Hybrid printing technology provides an attractive alternative as it combines a cell‐free ink for providing mechanical support with a bioink for housing embedded cells. Several hybrid printed structures have been developed, utilizing thermoplastic polymers such as polycaprolactone as structural support. These thermoplastics demonstrated limited structural integration with the cell‐laden components, and this may compromise the overall performance. In this work, a hybrid printing platform is presented using two distinct hydrogel inks that share the same photo‐crosslinking chemistry to enable simple fabrication and seamless structural integration. A mechanically reinforced hydrogel ink is developed comprising cellulose nanocrystals and gelatin methacryloyl/hyaluronic acid methacrylate (GelMA/HAMA) as the structural component, and GelMA/HAMA as the cytogel containing a mouse chondrogenic cell line, ATDC5. Hybrid printed constructs with encapsulated cells are fabricated using the two optimized inks, and the structural integration of the constructs is evaluated by cyclic mechanical compression. Finally, the cell viability of encapsulated ATDC5 cells in the hybrid printed structures is evaluated.

中文翻译：

使用纤维素纳米晶体增强的GelMA / HAMA水凝胶进行混合印刷以改善结构整合

像软组织一样具有适当机械性能的软组织的3D打印仍然是一个挑战。混合打印技术提供了一种有吸引力的选择，因为它结合了无细胞墨水和生物墨水，以提供机械支撑，而生物墨水用于容纳嵌入式细胞。利用热塑性聚合物例如聚己内酯作为结构载体，已经开发了几种混合印刷结构。这些热塑性塑料与含泡孔组件的结构整合有限，这可能会损害整体性能。在这项工作中，提出了一种混合印刷平台，该平台使用两种截然不同的水凝胶油墨，它们共享相同的光交联化学，以实现简单的制造和无缝的结构集成。开发了一种机械增强的水凝胶油墨，其中包含纤维素纳米晶体和明胶甲基丙烯酰基/透明质酸甲基丙烯酸酯（GelMA / HAMA）作为结构成分，而GelMA / HAMA作为包含小鼠软骨细胞系ATDC5的细胞凝胶。使用两种优化的墨水制造带有包封细胞的混合印刷构建体，并通过循环机械压缩评估构建体的结构整合。最后，评估了混合印刷结构中封装的ATDC5细胞的细胞活力。通过循环机械压缩评估构造体的结构整合。最后，评估了混合印刷结构中封装的ATDC5细胞的细胞活力。通过循环机械压缩评估构造体的结构整合。最后，评估了混合印刷结构中封装的ATDC5细胞的细胞活力。

更新日期：2020-12-16

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