Curcuma longa and Curcumin have been documented to have a wide spectrum of pharmacological effects, including anti-Acanthamoeba activity. Hence, this study sought to explore the anti-adhesion activity of C. longa extract and Curcumin against Acanthamoeba triangularis trophozoites and cysts in plastic and contact lenses. Our results showed that C. longa extract and Curcumin significantly inhibited the adhesion of A. triangularis trophozoites and cysts to the plastic surface, as investigated by the crystal violet assay (P < 0.05). Also, an 80–90% decrease in adhesion of trophozoites and cysts to the plastic surface was detected following the treatment with C. longa extract and Curcumin at 1/2 × MIC, compared to the control. In the contact lens model, approximately 1 log cells/mL of the trophozoites and cysts was reduced when the cells were treated with Curcumin, when compared to the control. Pre-treatment of the plastic surface with Curcumin at 1/2-MIC reduced 60% and 90% of the adhesion of trophozoites and cysts, respectively. The reduction in 1 Log cells/mL of the adhesion of A. triangularis trophozoites was observed when lenses were pre-treated with both the extract and Curcumin. Base on the results obtained from this study, A. triangularis trophozoites treated with C. longa extract and Curcumin have lost strong acanthopodia, thorn-like projection pseudopodia observed by scanning electron microscope. This study also revealed the therapeutic potentials of C. longa extract and Curcumin, as such, have promising anti-adhesive potential that can be used in the management/prevention of A. triangularis adhesion to contact lenses.

姜黄和姜黄素已被证明具有广泛的药理作用，包括抗棘阿米巴活性。因此，本研究试图探索长叶假单胞菌提取物和姜黄素对三角棘阿米巴滋养体和塑料和隐形眼镜中的包囊的抗粘附活性。我们的结果表明，通过结晶紫试验研究， C. longa提取物和姜黄素显着抑制了A. triangularis滋养体和包囊对塑料表面的粘附 (P < 0.05)。此外，在用C. longa处理后，检测到滋养体和包囊对塑料表面的粘附减少了 80-90%与对照相比，提取物和姜黄素的浓度为 1/2 × MIC。在隐形眼镜模型中，与对照相比，当用姜黄素处理细胞时，滋养体和囊肿减少了大约 1 log 细胞/mL。用 1/2-MIC 的姜黄素预处理塑料表面分别减少了 60% 和 90% 的滋养体和包囊的粘附。当镜片用提取物和姜黄素进行预处理时，观察到A. triangularis滋养体的粘附减少 1 Log 细胞/mL 。根据本研究获得的结果，用C. longa处理的A. triangularis滋养体提取物和姜黄素已失去强棘足，扫描电镜观察到刺状突起伪足。该研究还揭示了长叶仙人掌提取物和姜黄素的治疗潜力，因此，具有良好的抗粘连潜力，可用于管理/预防三角仙人掌与隐形眼镜的粘连。