Journal of Proteomics ( IF 2.8 ) Pub Date : 2020-11-17 , DOI: 10.1016/j.jprot.2020.104050 Renwen Zheng 1 , Yuxian Xia 1 , Nemat O Keyhani 2
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The narrow host range entomopathogenic fungus, Metarhizium acridum, is an environmentally friendly acridid specific pathogen used for locust control. The locust is capable of responding within hours of infection, however, little is known concerning how the locust detects the pathogen. Here, we have identified 3213 proteins in the infected antennal proteome of the migratory locust, Locusta migratoria. iTRAQ comparative analyses of antennal proteomes identified 194 differentially abundant proteins (DAPs) between the uninfected and infected males, 218 DAPs between the uninfected and infected females, and 240 DAPs between infected males and infected females. In relation to olfaction, a total of 29 chemosensory proteins (CSPs), 9 odorant binding proteins (OBPs), 31 odorant receptors (ORs), and 8 ionotropic receptors (IRs) were differentially abundant after M. acridum infection, with a subset of 12 proteins found in both infected male and female antennae not present in uninfected individuals. The time course of the gene expression profiles of olfaction related DAPs were investigated by quantitative real-time PCR (qRT-PCR). Our data indicate significant changes in the antennal proteomes of male and female locusts in response to a microbial pathogen, highlighting the potential participation of olfactory processes in pathogen detection and response.
Biological significance
The ability of an organism to detect microbial pathogens is essential for mounting a response to mitigate the spread of the infection. Using iTRAQ-based proteomic analyses changes in the protein repertoire of the antennae of male and female locusts in response to infection by a host-specific pathogen were determined. These data show proteomic alterations that are also sex-specific, identifying members of olfactory pathways that are modified in response to infection. Our data identify antennal and related olfactory proteins that are candidates for mediating host detection of pathogens, and that may contribute to subsequent behavioral and/or immune responses of the host to the infection challenge.
中文翻译：
雄性和雌性飞蝗触角蛋白质组对真菌病原体感染的不同反应
寄主范围窄的昆虫病原真菌绿僵菌是一种用于防治蝗虫的环境友好型刺槐特异性病原体。蝗虫能够在感染后数小时内做出反应，然而，关于蝗虫如何检测病原体知之甚少。在这里，我们在受感染的迁徙蝗虫（ Locusta migratoria）的触角蛋白质组中鉴定了 3213 种蛋白质. iTRAQ 对触角蛋白质组的比较分析确定了未感染和感染男性之间的 194 种差异丰富的蛋白质 (DAP)，未感染和感染女性之间的 218 种 DAP，以及感染男性和感染女性之间的 240 种 DAP。在嗅觉方面，在M. acridum 之后，共有 29 种化学感觉蛋白 (CSP)、9 种气味结合蛋白 (OBP)、31 种气味受体 (OR) 和 8 种离子型受体 (IR) 差异丰富感染，在受感染的雄性和雌性触角中发现的 12 种蛋白质的子集在未感染的个体中不存在。通过定量实时PCR（qRT-PCR）研究嗅觉相关DAP的基因表达谱的时间过程。我们的数据表明雄性和雌性蝗虫的触角蛋白质组对微生物病原体的反应发生了显着变化，突出了嗅觉过程在病原体检测和反应中的潜在参与。
生物学意义
生物体检测微生物病原体的能力对于采取应对措施以减轻感染传播至关重要。使用基于 iTRAQ 的蛋白质组学分析，确定了响应宿主特异性病原体感染的雄性和雌性蝗虫触角蛋白质库的变化。这些数据显示了也是性别特异性的蛋白质组学改变，识别了响应感染而改变的嗅觉通路的成员。我们的数据确定了触角和相关的嗅觉蛋白，它们是介导宿主检测病原体的候选者，并且可能有助于宿主随后对感染挑战的行为和/或免疫反应。
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