Abstract In this study, the start-up of anammox in a sequencing batch reactor (SBR) using conventional activated and anaerobic sludge in 1:1 ratio (v/v) as inoculum was achieved using an unconventional strategy. The ratio of NH4+-N to NO2−–N in the influent was not maintained as per the stoichiometry (1:1.32) of anammox and rather varied depending on the effluent characteristics. Nitrite was omitted during its accumulation period to avoid its toxicity during the start-up phase. The SBR having polyurethane foam impregnated with activated carbon (Levapor® carriers) was operated for 212 d (temperature, 37 °C; influent pH, 8). Anammox process was started up in ~95 d with 85% average total nitrogen (TN) removal. Thereafter, the reactor showed average TN removal efficiency of 85% and exhibited an average NH4+-N and NO2−–N removals of 99.9% each at maximum influent NH4+-N and NO2−–N concentrations of 200 mg L−1 and 264 mg L−1, respectively. The 16S rRNA gene amplicon sequencing analysis of suspended sludge samples (100 d, 150 d, and 186 d) from the reactor revealed the dominance of infrequently occurring anammox bacterial genus Candidatus Jettenia in the reactor. The phyla Chloroflexi, Proteobacteria, and Bacteriodetes were also detected in the reactor.

中文翻译：

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使用非常规策略在序批式反应器中从活性污泥和厌氧污泥成功启动厌氧氨氧化工艺

摘要 在本研究中，采用非常规策略，以 1:1 比例 (v/v) 的常规活性污泥和厌氧污泥作为接种物，在序批式反应器 (SBR) 中启动厌氧氨氧化。进水中 NH4+-N 与 NO2--N 的比率不是按照厌氧氨氧化的化学计量比 (1:1.32) 保持的，而是根据出水特性而变化。亚硝酸盐在其积累期间被省略以避免其在启动阶段的毒性。具有用活性炭（Levapor® 载体）浸渍的聚氨酯泡沫的 SBR 运行 212 天（温度，37 °C；进水 pH 值，8）。厌氧氨氧化工艺在约 95 天后启动，平均总氮 (TN) 去除率为 85%。此后，反应器的平均 TN 去除效率为 85%，平均 NH4+-N 和 NO2--N 去除率为 99。在最大进水 NH4+-N 和 NO2--N 浓度分别为 200 mg L-1 和 264 mg L-1 时，各为 9%。来自反应器的悬浮污泥样品（100 天、150 天和186 天）的16S rRNA 基因扩增子测序分析揭示了反应器中不常出现的厌氧氨氧化细菌属Candidatus Jettenia 的优势。在反应器中也检测到了 Chloroflexi、Proteobacteria 和 Bacteriodetes。