As an emerging field, DNA nanotechnology has been applied to the fabrication of drug delivery systems. Unprecedented spatial addressability and intrinsic sequence encoding enable DNA strands to self-assemble into well-defined 2D and 3D DNA nanostructures with specifically controlled sizes, shapes and surface charges. Multifunctional DNA nanostructures have been created and applied as promising platforms for drug delivery, imaging, and theranostics. Advantages of chemotherapy, gene therapy, and immunotherapy, among others, have been integrated into such functional nanodevices, showing potential in tumor-targeted therapy and diagnosis. In this review, we summarize general methods for the construction of DNA nanodevices and focus on targeting strategies favored by the compatibility of DNA nanotechnology. Additionally, we highlight the outlook and challenges facing the use of DNA nanotechnology in cancer therapy.

作为一个新兴领域，DNA纳米技术已被应用于药物递送系统的制造。前所未有的空间寻址能力和内在序列编码使 DNA 链能够自组装成定义明确的 2D 和 3D DNA 纳米结构，并具有特别控制的尺寸、形状和表面电荷。多功能 DNA 纳米结构已被创建并用作药物输送、成像和治疗诊断学的有前景的平台。化学疗法、基因疗法和免疫疗法等的优势已被整合到此类功能性纳米装置中，在肿瘤靶向治疗和诊断中显示出潜力。在这篇综述中，我们总结了构建 DNA 纳米器件的一般方法，并重点关注 DNA 纳米技术兼容性所青睐的靶向策略。此外，