In this study, primary and immortalized bovine intestinal epithelial cells (BIECs) were characterized for the expression of surface carbohydrate moieties. Primary BIEC-c4 cells showed staining greater than 90 % for 16 lectins but less than 50 % staining for four lectins. Immortalized BIECs showed significantly different lectin binding profile for few lectins compared to BIEC-c4 cells. BIEC-c4 cells were studied for infectivity to E. coli, Salmonella enterica, bovine rotavirus, bovine coronavirus, and bovine viral diarrhea virus. Bovine strain E. coli B41 adhered to BIEC-c4 cells and Salmonella strains S. Dublin and S. Mbandaka showed strong cell invasion. BIEC-c4 cells were susceptible to bovine rotavirus. LPS stimulation upregulated IL-10, IL-8, and IL-6 expression and Poly I:C upregulated TLR 8 and TLR 9 expression. This study provides important knowledge on the glycoconjugate expression profile of primary and immortalized BIECs and infectivity and immune responses of primary BIECs to bacterial and viral pathogens or ligands.

在这项研究中，原代和永生的牛肠上皮细胞（BIECs）的表面碳水化合物部分的表达进行了表征。BIEC-c4原代细胞显示16种凝集素染色大于90％，但四种凝集素染色小于50％。与BIEC-c4细胞相比，永生化的BIEC对几种凝集素显示出明显不同的凝集素结合特性。研究了BIEC-c4细胞对大肠杆菌，肠炎沙门氏菌，牛轮状病毒，牛冠状病毒和牛病毒性腹泻病毒的感染性。牛菌株大肠杆菌B41粘附到BIEC -C 4细胞和沙门氏菌菌株S.都柏林和S.姆班达卡显示出强烈的细胞侵袭。BIEC-c4细胞易感染牛轮状病毒。LPS刺激上调了IL-10，IL-8和IL-6的表达，而Poly I：C上调了TLR 8和TLR 9的表达。这项研究提供了有关原发性和永生化BIECs的糖缀合物表达谱以及原发性BIECs对细菌和病毒病原体或配体的感染性和免疫反应的重要知识。