当前位置: X-MOL 学术BBA Gen. Subj. › 论文详情
Our official English website, www.x-mol.net, welcomes your feedback! (Note: you will need to create a separate account there.)
Transport processes in equine oocytes and ovarian tissue during loading with cryoprotective solutions
Biochimica et Biophysica Acta (BBA) - General Subjects ( IF 3 ) Pub Date : 2020-11-17 , DOI: 10.1016/j.bbagen.2020.129797
Jürgen Lotz , Sercan Içli , Dejia Liu , Sükrü Caliskan , Harald Sieme , Willem F. Wolkers , Harriëtte Oldenhof

Background

Rational design of cryopreservation strategies for oocytes and ovarian cortex tissue requires insights in the rate at which cryoprotective agents (CPA) permeate and concomitant water transport takes place. The aim of the current study was to investigate possible differences in permeation kinetics of different CPAs (i.e., glycerol/GLY, ethylene glycol/EG, dimethyl sulfoxide/DMSO, and propylene glycol/PG), in equine oocytes as well as ovarian tissue.

Methods

Membrane permeability of oocytes to water (Lp) and to CPAs (Ps) was inferred from video microscopic imaging of oocyte volume responses during perfusion with anisotonic and CPA solutions. CPA diffusion into ovarian tissue and tissue dehydration was monitored during incubation, using osmometer and weight measurements, to derive CPA diffusion coefficients (D).

Results

Membrane permeability of oocytes towards CPAs was found to increase in the order GLY < EG < DMSO<PG. Permeability towards water in anisotonic solutions was determined to be higher than in CPA solutions, indicating CPAs alter membrane permeability properties. CPA diffusion in ovarian tissue increased in the order GLY,PG < EG,DMSO. Tissue dehydration was found to increase with exposure to increasing CPA concentrations, which inversely correlated with CPA diffusivity.

Conclusions

In conclusion, it is shown here that the rate of CPA movement across membrane bilayers is determined by different physical barrier factors than those determining CPA movement in tissues.

General significance

The parameters presented in this study can be applied in models describing solute and water transport in cells and tissues, as well as in cryopreservation protocols.



中文翻译:

冷冻保护溶液加载过程中马卵母细胞和卵巢组织中的运输过程

背景

卵母细胞和卵巢皮质组织的冷冻保存策略的合理设计需要洞悉冷冻保护剂(CPA)渗透和伴随的水传输发生的速率。本研究的目的是研究马卵母细胞和卵巢组织中不同CPA(即甘油/ GLY,乙二醇/ EG,二甲基亚砜/ DMSO和丙二醇/ PG)的渗透动力学可能存在的差异。

方法

卵母细胞对水(Lp)和CPA(Ps)的膜通透性是通过在等渗和CPA溶液灌注过程中卵母细胞体积反应的视频显微镜成像得出的。在孵育期间,使用渗透压计和体重测量监测CPA扩散到卵巢组织和组织脱水中,以得出CPA扩散系数(D)。

结果

发现卵母细胞对CPA的膜通透性以GLY <EG <DMSO <PG的顺序增加。确定在各向异性溶液中对水的渗透性高于在CPA溶液中,表明CPA改变了膜的渗透性。CPA在卵巢组织中的扩散顺序为GLY,PG <EG,DMSO。发现组织脱水随着暴露于CPA浓度增加而增加,这与CPA扩散率成反比。

结论

总之,这里表明,跨膜双层的CPA移动速率是由与确定组织中CPA移动的物理屏障因子不同的物理屏障因子决定的。

一般意义

这项研究中提出的参数可用于描述溶质和水在细胞和组织中的运输模型,以及在低温保存方案中的应用。

更新日期:2020-11-25
down
wechat
bug