The efficient operation of an algal photobioreactor (PBR) requires appropriate lighting conditions. Despite recent advantages in PBR design and operation, optimal lighting conditions are still not fully understood. This work focuses on the effect of light quality and culturing temperature on the algal growth kinetics and lipid content. The green alga Chlamydomonas reinhardtii was grown in batch culture in a constant-temperature incubator-shaker under LED light. Three light wavelength regimes were studied: blue light (peaking at 433 nm–447 nm, 458 nm–470 nm), white-yellow light (peaking at 456–458 nm in blue, 545 nm in green and 570 nm in yellow) and red-orange light (peaking at 580 nm–594 nm and 604 nm in yellow-orange, 630 nm and 656 nm in red, and 735 nm in IR). Results show that red-orange light obtained 38% higher biomass productivity than blue light at 24 °C, and blue light obtained 13% higher biomass productivity than red-orange light at 32 °C. Red-orange light or temperatures of 30–32 °C favored lipid accumulation with a lipid mass fraction and lipid concentration of 44% and 80% higher, respectively, compared to blue light at 24 °C. In the late exponential phase, when nutrients in the medium were still sufficient, light quality was the predominant factor controlling the lipid synthesis and accumulation. While in the late stationary phase under nutrient starvation, a strong interactive effect was detected between the light quality and temperature (P = 0.02) for lipid synthesis and accumulation. Under both temperature and nutrient stress, there was a reduced effect of the light quality on the lipid synthesis.

藻类光生物反应器（PBR）的有效运行需要适当的照明条件。尽管最近在PBR设计和操作中有优势，但最佳照明条件仍未完全了解。这项工作的重点是光照质量和培养温度对藻类生长动力学和脂质含量的影响。绿藻衣藻在恒温培养箱中在LED灯下分批培养。研究了三种光波长范围：蓝光（峰值在433 nm–447 nm，458 nm–470 nm），白黄光（峰值在456-458 nm，蓝色，绿色545 nm，黄色570 nm）和橘红色的光（在橘黄色中为580 nm–594 nm和604 nm峰值，红色为630 nm和656 nm，红外为735 nm）。结果显示，在24°C时，橘红色的光的生物量生产率比蓝光高38％，在32°C时，蓝光的生物量生产率比蓝橙色高13％。与24°C的蓝光相比，橙红色的光或30-32°C的温度有利于脂质积聚，脂质质量分数和脂质浓度分别高出44％和80％。在指数后期 当培养基中的营养仍然充足时，光照质量是控制脂质合成和积累的主要因素。在营养物饥饿的静止后期，在光质量和温度之间检测到强烈的交互作用（P  = 0.02）用于脂质合成和积累。在温度和营养胁迫下，光质量对脂质合成的影响降低。