Self-assembling peptide hydrogels have a high water content, good biocompatibility and have become a competitive research object in the fields of tissue engineering, cancer treatment and drug delivery. In our research, a hexapeptide with high pH sensitivity was designed and synthesized by utilizing a solid-phase synthesis method. Under physiological conditions, the peptide could self-assemble into a hydrogel. When it reached the tumor acidic microenvironment, the peptide was degraded and doxorubicin was released to exert its antitumor effect. A series of physicochemical properties were investigated, including gelling ability, secondary structure, micromorphology, rheological properties and drug release studies. The results illustrated that PIDO peptide hydrogel has good pH responsiveness and injectability. In vitro cytotoxicity experiments and in vivo antitumor experiments showed that PIDO peptide hydrogel has a highly effective therapeutic effect on tumor cells and is less toxic to normal tissues. Our research provides a promising option for targeted drug delivery and sustainable release.

中文翻译：

---

使用智能阿霉素加载的六肽水凝胶对乳腺癌进行靶向化疗。

自组装肽水凝胶具有高水含量，良好的生物相容性，并且已成为组织工程，癌症治疗和药物递送领域中的竞争性研究对象。在我们的研究中，设计并利用固相合成方法合成了具有高pH敏感性的六肽。在生理条件下，该肽可自组装成水凝胶。当到达肿瘤酸性微环境时，该肽被降解并释放阿霉素以发挥其抗肿瘤作用。研究了一系列理化性质，包括胶凝能力，二级结构，微观形态，流变性质和药物释放研究。结果表明PIDO肽水凝胶具有良好的pH响应性和注射性。体外细胞毒性实验和体内抗肿瘤实验表明，PIDO肽水凝胶对肿瘤细胞具有高度有效的治疗作用，并且对正常组织的毒性较小。我们的研究为靶向药物的递送和可持续释放提供了有希望的选择。