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Design and Construction of Enzyme–Nanozyme Integrated Catalyst as a Multifunctional Detection Platform
Industrial & Engineering Chemistry Research ( IF 3.8 ) Pub Date : 2020-11-16 , DOI: 10.1021/acs.iecr.0c04094 Junyang Xu 1 , Huajiao Liu 1 , Jianqiao Liu 1 , Ying He 1 , Jing Gao 1 , Jiafu Shi 2 , Yanjun Jiang 1, 3
Industrial & Engineering Chemistry Research ( IF 3.8 ) Pub Date : 2020-11-16 , DOI: 10.1021/acs.iecr.0c04094 Junyang Xu 1 , Huajiao Liu 1 , Jianqiao Liu 1 , Ying He 1 , Jing Gao 1 , Jiafu Shi 2 , Yanjun Jiang 1, 3
Affiliation
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A new integrated catalyst, Fe3O4@Cu/GMP–GOx, was successfully constructed by assembling Cu2+ and guanosine 5-monophosphate (GMP) on magnetic nanoparticles and in situ encapsulating glucose oxidase (GOx). As the nanozyme, Fe3O4@Cu/GMP has the dual response of catalase- and laccase-like activity, which can effectively improve the cascade catalytic efficiency and amplify the colorimetric detection signal. Compared with free enzymes, Fe3O4@Cu/GMP and Fe3O4@Cu/GMP–GOx showed enhanced thermal, mechanical, and storage stability. Fe3O4@Cu/GMP can be applied to the detection of H2O2, with a linear response range of 0 to 198 μM and a limit of detection (LOD) of 0.477 μM. Fe3O4@Cu/GMP–GOx can be applied to the detection of glucose and β-arbutin and showed good selectivity and anti-interference performance. For glucose, the linear response range was 0 to 800 μM, and the LOD was 5.516 μM. For arbutin, the absorbance was linearly correlated with the concentration of arbutin in the range of 0 to 60 μM and 100 to 400 μM, and the LOD was 0.766 μM. This integrated catalyst provides a simple strategy for the application of an enzyme–nanozyme cascade system in colorimetric detection.
中文翻译：
酶-纳米酶集成催化剂多功能检测平台的设计与构建
通过在磁性纳米粒子上组装Cu 2+和鸟苷5-一磷酸酯（GMP）并原位包封葡萄糖氧化酶（GOx），成功构建了一种新型的集成催化剂Fe 3 O 4 @ Cu / GMP-GOx 。作为纳米酶，Fe 3 O 4 @ Cu / GMP具有过氧化氢酶和漆酶样活性的双重响应，可以有效地提高级联催化效率并放大比色检测信号。与游离酶相比，Fe 3 O 4 @ Cu / GMP和Fe 3 O 4 @ Cu / GMP-GOx显示出增强的热，机械和存储稳定性。铁3 O 4@ Cu / GMP可应用于H 2 O 2的检测，线性响应范围为0至198μM，检测极限（LOD）为0.477μM。Fe 3 O 4 @ Cu / GMP-GOx可用于葡萄糖和β-熊果苷的检测，具有良好的选择性和抗干扰性能。对于葡萄糖，线性响应范围为0至800μM，LOD为5.516μM。对于熊果苷，吸光度与熊果苷浓度在0至60μM和100至400μM的范围内线性相关，LOD为0.766μM。这种集成的催化剂为酶-纳米酶级联系统在比色检测中的应用提供了一种简单的策略。
更新日期：2020-11-25
中文翻译：
酶-纳米酶集成催化剂多功能检测平台的设计与构建
通过在磁性纳米粒子上组装Cu 2+和鸟苷5-一磷酸酯（GMP）并原位包封葡萄糖氧化酶（GOx），成功构建了一种新型的集成催化剂Fe 3 O 4 @ Cu / GMP-GOx 。作为纳米酶，Fe 3 O 4 @ Cu / GMP具有过氧化氢酶和漆酶样活性的双重响应，可以有效地提高级联催化效率并放大比色检测信号。与游离酶相比，Fe 3 O 4 @ Cu / GMP和Fe 3 O 4 @ Cu / GMP-GOx显示出增强的热，机械和存储稳定性。铁3 O 4@ Cu / GMP可应用于H 2 O 2的检测，线性响应范围为0至198μM，检测极限（LOD）为0.477μM。Fe 3 O 4 @ Cu / GMP-GOx可用于葡萄糖和β-熊果苷的检测，具有良好的选择性和抗干扰性能。对于葡萄糖，线性响应范围为0至800μM，LOD为5.516μM。对于熊果苷，吸光度与熊果苷浓度在0至60μM和100至400μM的范围内线性相关，LOD为0.766μM。这种集成的催化剂为酶-纳米酶级联系统在比色检测中的应用提供了一种简单的策略。
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