Abstract In this paper, konjac oligoglucomannan (KOGM) was obtained with a hydrolysis rate of 56.24% by controlling the hydrolysis conditions. KOGM was passed through a 0.2 kDa dialysis bag, a 3 kDa ultrafiltration tube, and a 5 kDa ultrafiltration tube, creating samples with molecular weights of 0.2–3 kDa (IV), 3–5 kDa (III), and >5 kDa (II), respectively. The in vitro antioxidant activities of the KOGM samples were tested by measuring their removal effects on ˙OH, O 2 − {\text{O}}_{2}^{-} , and DPPH˙. The in vivo antioxidant activities of the samples were analyzed by measuring their impacts on the malondialdehyde (MDA) content, superoxide dismutase (SOD) activity, and glutathione peroxidase (GSH-PX) activity in mice. The results show that the KOGM samples in groups III and IV could effectively remove ˙OH, O 2 − {\text{O}}_{2}^{-} , and DPPH˙; the KOGM samples in all three groups could enhance the SOD and GSH-PX activities and reduce the MDA content in the liver tissues of mice; finally, the antioxidant activity of KOGM is negatively correlated with the molecular weight.

中文翻译：

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不同分子量魔芋寡葡甘露聚糖的制备及其体内外抗氧化活性

摘要 本文通过控制水解条件，获得了水解率为56.24%的魔芋低聚葡甘露聚糖(KOGM)。KOGM 通过 0.2 kDa 透析袋、3 kDa 超滤管和 5 kDa 超滤管，产生分子量为 0.2–3 kDa (IV)、3–5 kDa (III) 和 >5 kDa 的样品（ II)，分别。KOGM 样品的体外抗氧化活性通过测量它们对 ˙OH、O 2 − {\text{O}}_{2}^{-} 和 DPPH˙ 的去除效果来测试。通过测量样品对小鼠丙二醛 (MDA) 含量、超氧化物歧化酶 (SOD) 活性和谷胱甘肽过氧化物酶 (GSH-PX) 活性的影响来分析样品的体内抗氧化活性。结果表明，第三组和第四组的KOGM样品可以有效去除˙OH、O 2 − {\text{O}}_{2}^{-} 和DPPH˙；三组KOGM样品均能提高小鼠肝组织中SOD和GSH-PX的活性，降低MDA含量；最后，KOGM 的抗氧化活性与分子量呈负相关。