ABSTRACT

Guided bone regeneration (GBR) is an effective approach for oral and maxillofacial surgery. The membranes were fabricated by particle deposition into layer-by-layer of silk fibroin (SF)/poly (vinyl alcohol) (PVA)/silk fibroin (SF). These membranes were varied amount of PVA: 0, 100, 200, and 300 ml of 5% (w/v) PVA (control, PVA100, 200, and 300, respectively). Morphology and molecular structure were explored and characterised by scanning electron microscopy, Fourier transform infrared spectroscopy, and differential scanning calorimetry, respectively. The membranes were tested physical and mechanical properties. They were cultured with osteoblasts for evaluation of the biological performances: cell viability and proliferation, alkaline phosphatase activity, calcium content, amount of protein, and calcium deposition. PVA100, 200, and 300 had different coarse-grained surfaces, molecular organisations, structural stabilities and properties. PVA300 had the morphology, molecular organisation, and structural stability that lead to suitable physical and mechanical properties to enhance the biological performance. PVA300 is a promising candidate for GBR.

摘要

引导骨再生（GBR）是口腔颌面外科的有效方法。通过颗粒沉积到丝素蛋白 (SF)/聚 (乙烯醇) (PVA)/丝素蛋白 (SF) 的逐层中来制造膜。这些膜的 PVA 含量不同：0、100、200 和 300 ml 的 5% (w/v) PVA（对照，分别为 PVA100、200 和 300）。形态学和分子结构分别通过扫描电子显微镜、傅里叶变换红外光谱和差示扫描量热法进行了探索和表征。测试膜的物理和机械性能。将它们与成骨细胞一起培养以评估生物学性能：细胞活力和增殖、碱性磷酸酶活性、钙含量、蛋白质含量和钙沉积。PVA100、200、和 300 具有不同的粗粒表面、分子组织、结构稳定性和性能。PVA300 具有形态、分子组织和结构稳定性，可产生合适的物理和机械性能，从而提高生物性能。PVA300 是 GBR 的有希望的候选者。