ABSTRACT

The Chinese water chestnut (Eleocharis dulcis (Brum.f.) Trin.) is conventionally propagated by underground corm with a lower propagation rate. In the present study, we attempted to develop a simple and efficient protocol for in vitro shoot regeneration, corm formation, and the generation of plantlets, on MS medium supplemented with BA, TDZ, NAA, and IAA. Additionally, the effect of BA on the expression of EdIPT and EdCKX genes was determined. The highest number of shoots (10.9) was produced by 0.5 mg/L BA, while that was the 0.1 mg/L TDZ (11.9), followed by the combination of 0.1 mg/L TDZ and 0.5 mg/L IAA (10.6), and shoot produced root spontaneously in these treatments. 60 g/L of sucrose was optimal for inducing the shoots while the highest corm number (5.8) was achieved by 90 g/L sucrose. 1.0 mg/L BA was optimal for corm formation with the highest number of corm (3.5) and the largest diameter (7.4 mm). NAA or IAA at 0.1 mg/L was optimal for rooting. Furthermore, we found the expression of EdIPT gene was up-regulated by the application of BA at a relatively lower level, and a high concentration of BA promoted expression of EdCKX gene.

摘要

water（Eleocharis dulcis（Brum.f.）Trin。）通常通过地下球茎繁殖，繁殖率较低。在本研究中，我们尝试在补充BA，TDZ，NAA和IAA的MS培养基上开发一种简单有效的体外芽再生，球茎形成和小植株生成的方案。此外，BA对EdIPT和EdCKX表达的影响基因已确定。0.5 mg / L BA产生最多的芽（10.9），而0.1 mg / L TDZ（11.9）最多，其次是0.1 mg / L TDZ和0.5 mg / L IAA（10.6），并在这些处理中自发产生根。60 g / L蔗糖最适合诱导芽，而90 g / L蔗糖可达到最高球茎数（5.8）。1.0 mg / L BA最适合形成球茎，球茎数量最多（3.5），直径最大（7.4 mm）。0.1 mg / L的NAA或IAA最适合生根。此外，我们发现表达EdIPT基因是由BA的应用在一个相对较低的水平上调，和BA的高浓度促进了表达EdCKX基因。