BACKGROUND In Acre state, Brazil, the dissemination of cutaneous leishmaniasis has increased in recent years, with limited knowledge of the potential Leishmania spp. vectors involved. OBJECTIVES Here, data concerning the sandfly fauna of Brasiléia municipality, Leishmania DNA-detection rates and the identification of blood meal sources of insects captured in 2013-2015 are presented. METHODS Parasite detection in female sandflies was performed individually by multiplex polymerase chain reaction (PCR) (Leishmania kDNA/sandfly cacophony-gene), with the identification of Leishmania spp. by hsp70-PCR and sequencing. The identification of blood gut-content from fed females was performed by cyt b-PCR and sequencing. FINDINGS A total of 4,473 sandflies were captured. A subgroup of 864 non-blood-fed females evaluated for the presence of Leishmania DNA showed 2.9% positivity for Leishmania (Viannia) braziliensis and L. (V.) guyanensis. The identification of blood meal sources was performed in 96 blood-fed females, allowing the identification of 13 vertebrate species. In nine/96 fed females, DNA from L. (V.) shawi, L. (V.) guyanensis, L. (V.) braziliensis and Endotrypanum sp. was detected. MAIN CONCLUSIONS In Brumptomyia sp. and Evandromyia termitophila, the first report of Leishmania DNA-detection is provided in Acre; Nyssomyia shawi is implicated as potential vector of L. (V.) braziliensis and L. (V.) guyanensis for the first time in Brazil.

中文翻译：

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巴西亚马逊流域阿卡州白蛉动物群（双翅目：Psychodidae）概述，随后进行利什曼原虫 DNA 检测和血粉鉴定


背景 在巴西阿卡州，近年来皮肤利什曼病的传播有所增加，但对潜在的利什曼原虫属的了解有限。涉及的向量。目标 这里提供了 2013-2015 年捕获的巴西白蛉动物群、利什曼原虫 DNA 检测率和昆虫血粉来源鉴定的数据。方法通过多重聚合酶链式反应（PCR）（利什曼原虫kDNA/白蛉杂音基因）单独进行雌性白蛉寄生虫检测，并鉴定利什曼原虫属。通过 hsp70-PCR 和测序。通过细胞色素 b-PCR 和测序对进食雌性的血液肠道内容物进行鉴定。结果 总共捕获了 4,473 只白蛉。对 864 名非吸血女性的亚组进行了利什曼原虫 DNA 检测，结果显示，巴西利什曼原虫 (Viannia) 和圭亚那利什曼原虫 (Viannia) 阳性率为 2.9%。对 96 只吸血雌性进行了血粉来源的鉴定，从而鉴定了 13 种脊椎动物。在 9/96 喂养的雌性中，来自 L. (V.) shawi、L. (V.) Guyanensis、L. (V.) braziliensis 和 Endotrypanum sp. 的 DNA。被检测到。主要结论 Brumptomyia sp。和 Evandromyia termitophila，阿卡提供了第一份利什曼原虫 DNA 检测报告； Nyssomyia shawi 在巴西首次被认为是 L. (V.) braziliensis 和 L. (V.) Guyanensis 的潜在传播媒介。