Background Cassava pulp is a promising starch-based biomasses, which consists of residual starch granules entrapped in plant cell wall containing non-starch polysaccharides, cellulose and hemicellulose. Strain CT4T, a novel mesophilic anaerobic bacterium isolated from soil collected from a cassava pulp landfill, has a strong ability to degrade polysaccharides in cassava pulp. This study explored a rarely described species within the genus Clostridium that possessed a group of cassava pulp-degrading enzymes. Methods A novel mesophilic anaerobic bacterium, the strain CT4T, was identified based on phylogenetic, genomic, phenotypic and chemotaxonomic analysis. The complete genome of the strain CT4T was obtained following whole-genome sequencing, assembly and annotation using both Illumina and Oxford Nanopore Technology (ONT) platforms. Results Analysis based on the 16S rRNA gene sequence indicated that strain CT4T is a species of genus Clostridium. Analysis of the whole-genome average amino acid identity (AAI) of strain CT4T and the other 665 closely related species of the genus Clostridium revealed a separated strain CT4T from the others. The results revealed that the genome consisted of a 6.3 Mb circular chromosome with 5,664 protein-coding sequences. Genome analysis result of strain CT4T revealed that it contained a set of genes encoding amylolytic-, hemicellulolytic-, cellulolytic- and pectinolytic enzymes. A comparative genomic analysis of strain CT4T with closely related species with available genomic information, C. amylolyticum SW408T, showed that strain CT4T contained more genes encoding cassava pulp-degrading enzymes, which comprised a complex mixture of amylolytic-, hemicellulolytic-, cellulolytic- and pectinolytic enzymes. This work presents the potential for saccharification of strain CT4T in the utilization of cassava pulp. Based on phylogenetic, genomic, phenotypic and chemotaxonomic data, we propose a novel species for which the name Clostridium manihotivorum sp. nov. is suggested, with the type strain CT4T (= TBRC 11758T = NBRC 114534T).

中文翻译：

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木薯梭菌 sp. 11月，一种新型嗜温厌氧细菌，可产生木薯果肉降解酶


背景技术木薯浆是一种很有前途的淀粉基生物质，它由残留在植物细胞壁中的淀粉颗粒组成，含有非淀粉多糖、纤维素和半纤维素。 CT4T菌株是从木薯浆垃圾填埋场收集的土壤中分离出来的一种新型中温厌氧细菌，具有很强的降解木薯浆中多糖的能力。这项研究探索了梭菌属中一种很少被描述的物种，它拥有一组木薯果肉降解酶。方法基于系统发育、基因组、表型和化学分类学分析，鉴定出一种新型中温厌氧细菌——菌株CT4T。使用 Illumina 和牛津纳米孔技术 (ONT) 平台进行全基因组测序、组装和注释后获得了 CT4T 菌株的完整基因组。结果基于16S rRNA基因序列分析表明菌株CT4T为梭状芽胞杆菌属的一个种。对菌株 CT4T 和梭菌属其他 665 个密切相关物种的全基因组平均氨基酸同一性 (AAI) 的分析揭示了 CT4T 菌株与其他菌株的分离。结果显示，该基因组由一条 6.3 Mb 的环状染色体组成，包含 5,664 个蛋白质编码序列。菌株CT4T的基因组分析结果表明，它含有一组编码淀粉分解酶、半纤维素分解酶、纤维素分解酶和果胶分解酶的基因。对菌株 CT4T 与具有可用基因组信息的密切相关物种 C. amylolyticum SW408T 进行的比较基因组分析表明，菌株 CT4T 含有更多编码木薯果肉降解酶的基因，其中包括淀粉分解酶、半纤维素分解酶、纤维素分解酶和木薯分解酶的复杂混合物。果胶分解酶。 这项工作展示了菌株 CT4T 在木薯浆利用中糖化的潜力。基于系统发育、基因组、表型和化学分类学数据，我们提出了一个新物种，将其命名为 Clostridium manihotivorum sp。十一月建议使用型号菌株 CT4T (= TBRC 11758T = NBRC 114534T)。