Hydrogels have gained interest for use in tissue regeneration and wound healing because of their absorbing and swelling properties as well as their ability to mimic the natural extracellular matrix. Their use in wound healing specifically may be in the form of a patch or wound dressing or they may be administered within the wound bed as a filler, gel in situ, to promote healing. Thiolated hyaluronic acid‐polyethylene diacrylate (tHA‐PEGDA) hydrogels are ideal for this purpose due to their short gelation times at physiological temperature and pH. But these hydrogels alone are not enough and require added components to gain bioactivity. In this work, RGD adhesion peptides and an antivascular endothelial growth factor receptor‐2 (VEGF‐R2) DNA aptamer are incorporated into a tHA‐PEGDA hydrogel to make a bifunctional hyaluronic acid hydrogel. RGD peptides promote attachment and growth of cells while the anti‐VEGF‐R2 DNA aptamer seems to improve cell viability, induce cell migration, and spur the onset of angiogenesis by tube formation by endothelial cells. This bifunctional hydrogel supports cell culture and has improved biological properties. The data suggest that these hydrogels can be used for advanced tissue regeneration applications such as in wound healing.

中文翻译：

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抗 VEGF-R2 适体和 RGD 肽在双功能水凝胶中协同作用，增强血管生成潜力

水凝胶因其吸收和膨胀特性以及模仿天然细胞外基质的能力而在组织再生和伤口愈合中的应用引起了人们的兴趣。它们在伤口愈合中的用途具体可以是贴片或伤口敷料的形式，或者它们可以作为填充剂、原位凝胶施用在伤口床内以促进愈合。硫醇化透明质酸-聚乙二醇二丙烯酸酯 (tHA-PEGDA) 水凝胶非常适合此用途，因为它们在生理温度和 pH 值下的凝胶时间较短。但仅这些水凝胶是不够的，需要添加成分才能获得生物活性。在这项工作中，RGD 粘附肽和抗血管内皮生长因子受体-2 (VEGF-R2) DNA 适体被整合到 tHA-PEGDA 水凝胶中，制成双功能透明质酸水凝胶。RGD 肽促进细胞的附着和生长，而抗 VEGF-R2 DNA 适体似乎可以提高细胞活力，诱导细胞迁移，并通过内皮细胞形成管来刺激血管生成。这种双功能水凝胶支持细胞培养并具有改善的生物学特性。数据表明这些水凝胶可用于先进的组织再生应用，例如伤口愈合。