Much of our understanding of the cellular mechanisms underlying cnidarian‐algal symbiosis comes from studying the biological differences between the partners when they are engaged in symbiosis and when they are isolated from one another. When comparing the in hospite and ex hospite states in Symbiodiniaceae, the in hospite state is represented by algae sampled from hosts, and the ex hospite state is commonly represented by cultured algae. The use of cultured algae in this comparison may introduce nutrition as a confounding variable because, while hosts are kept in nutrient‐depleted conditions, culture media is nutrient rich and designed to facilitate algal growth. In this perspective, we reexamine how nutrition may be a confounding variable in studies that compare the biology of Symbiodiniaceae in hospite and in culture. We also suggest several innovations in experimental design to strengthen the comparison of the two lifestyles, including the adoption of nutritional controls, alternatives to culture for the representation of Symbiodiniaceae ex hospite, and the adoption of several proteomic approaches to find novel Symbiodiniaceae genes important for symbiosis.

中文翻译：

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使用养殖藻类研究念藻和藻类共生的局限性及未来研究的建议

我们对cnidarian-algal共生基础的细胞机制的大部分理解来自研究伴侣之间共生以及彼此分离时的生物学差异。当比较共生双齿科的临辉状态和临辉状态时，临辉状态由从宿主采样的藻类代表，而临辉状态通常由培养的藻类代表。在此比较中，使用养殖藻类可能会将营养作为一个混杂变量引入，因为虽然宿主处于营养耗尽状态，但培养基中营养丰富，旨在促进藻类生长。从这个角度出发，我们重新研究了营养如何可能成为在医院和文化中比较双生菌科生物学的混杂变量。