Metalaxyl and Metalaxyl-M are the fungicides that widely used in many countries. In this study, the environmental behaviors between metalaxyl and metalaxyl-M in Tubifex tubifex (T. tubifex) were quantitative analyzed by using a high performance liquid chromatography with photo-diode-array-detector (HPLC-DAD). Results demonstrated that there was no significant difference (p > 0.05) in the concentration of metalaxyl and metalaxyl-M in T. tubifex during the exposure process. However, the dissipation behaviors of metalaxyl and metalaxyl-M in T. tubifex were different (p < 0.05) during the non-exposure culture process. Meanwhile, the toxic effects were also evaluated by comparing the different influences of these two compounds on related physiological indicators, and functional enzyme activities. The survival rates of T. tubifex were 63.33 ± 15.28% (20 mg L⁻¹), 63.33 ± 5.77% (200 mg L⁻¹) treated with metalaxyl and were 50.00 ± 10.00% (20 mg L⁻¹), 46.67 ± 11.55 (200 mg L⁻¹) treated with metalaxyl-M at the non-exposure culture process. The autotomy rates were increased significantly compared with the initial in all treatments. Besides, the activities of CAT, SOD, and GST in T. tubifex were also inhibited by metalaxyl and metalaxyl-M treatments. Finally, the high-throughput transcriptome sequencing technology was applied to investigate the metabolic pathways of target analytes in T. tubifex, and results proved that the metabolic pathways associated with human diseases (such as viral myocarditis) were up-regulated expression for metalaxyl and metalaxyl-M treatments, and metalaxyl-M up-regulated more significantly. All the results demonstrated that metalaxyl-M had a higher toxicity than metalaxyl on T. tubifex.

甲霜灵和甲霜灵-M是在许多国家中广泛使用的杀菌剂。在这项研究中，通过使用具有光电二极管阵列检测器（HPLC-DAD）的高效液相色谱法定量分析了塔比菲（T.ifeifex）（T.tubifex）中甲霜灵和甲霜灵-M之间的环境行为。结果表明，没有显著差异（p  > 0.05）甲霜灵和精甲霜灵的在浓度T.水丝蚓在曝光过程期间。然而，甲霜灵和甲霜灵的消散行为在T.tubifex中是不同的（p <0.05）。同时，还通过比较这两种化合物对相关生理指标和功能酶活性的不同影响来评估其毒性作用。的存活率T.水丝蚓分别为63.33±15.28％ （ 20毫克的L ^-1），63.33±5.77％（200毫克的L ^-1与甲霜灵处理的）和分别为50 。在非接触式培养过程中，用甲霜灵-M处理了00±10.00％（20 mg L ^-1），46.67±11.55（200 mg L ^-1）。在所有治疗中，与最初相比，切开率显着提高。此外，CAT，SOD和GST在活动T.水丝蚓甲霜灵和甲霜灵-M处理也可抑制这些药物。最后，高通量转录组测序技术应用于调查目标的代谢途径的分析物在T.水丝蚓和结果证明了甲霜灵和精甲霜灵与人类疾病（如病毒性心肌炎）相关联的代谢途径是上调的表达-M处理和甲霜灵-M上调更为显着。所有结果表明，甲霜灵-M比甲霜灵对T.tubifex具有更高的毒性。