In the present study, the complete mitogenome of Clavaria fumosa, was sequenced, assembled, and compared. The complete mitogenome of C. fumosa is 256,807 bp in length and is the largest mitogenomes among all Basidiomycota mitogenomes reported. Comparative mitogenomic analysis indicated that the C. fumosa mitogenome contained the most introns and intronic ORFs among all fungal mitogenomes. Large intergenic regions, intronic regions, accumulation of repeat sequences and plasmid-derived genes together promoted the size expansion of the C. fumosa mitogenome. In addition, the rps3 gene was found subjected to positive selection between some Agaricales species. We found frequent intron gain/loss events in Agaricales mitogenomes, and four novel intron classes were detected in the C. fumosa mitogenome. Large-scale gene rearrangements were found occurred in Agaricales species and the C. fumosa mitogenome had a unique gene arrangement which differed from other Agaricales species. Phylogenetic analysis for 76 Basidiomycetes based on combined mitochondrial gene sets indicated that mitochondrial genes could be used as effective molecular markers for reconstructing evolution of Basidiomycota. The study served as the first report on the mitogenomes of the family Clavariaceae, which will promote the understanding of the genetics, evolution and taxonomy of C. fumosa and related species.

中文翻译：

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Clavaria fumosa的256 kb线粒体基因组是Basidiomycota门中最大的，并且富含内含子和内含子ORF

在本研究中，对Clavaria fumosa的完整有丝分裂基因组进行了测序，组装和比较。C. fumosa的完整有丝分裂基因组的长度为256,807 bp，是所有报道的Basidiomycota有丝分裂基因组中最大的有丝分裂基因组。对比线粒体基因组分析表明，在所有真菌有丝分裂基因组中，C。fumosa有丝分裂基因组包含最多的内含子和内含子ORF。大的基因间区域，内含子区域，重复序列的积累和质粒衍生的基因共同促进了C. fumosa有丝分裂基因组的大小扩展。另外，发现rps3基因在某些蛤gar属菌种之间进行正选择。我们发现在Agaricales有丝分裂基因组中频繁发生内含子增/减事件，并且在C. fumosa有丝分裂基因组中检测到四个新的内含子类别。发现大型基因重排发生在蛤gar菌种中，并且C. fumosa有丝分裂基因组具有独特的基因排列，这不同于其他蛤gar菌种。基于组合线粒体基因集的76担子菌的系统发育分析表明，线粒体基因可以用作重建担子菌进化的有效分子标记。该研究报告是关于克拉科的有丝分裂基因组的第一份报告，这将促进人们对C. fumosa和相关物种的遗传，进化和分类学的理解。基于组合线粒体基因集的76担子菌的系统发育分析表明，线粒体基因可以用作重建担子菌进化的有效分子标记。该研究报告是关于克拉科的有丝分裂基因组的第一份报告，这将促进人们对C. fumosa和相关物种的遗传，进化和分类学的理解。基于组合线粒体基因集的76担子菌的系统发育分析表明，线粒体基因可以用作重建担子菌进化的有效分子标记。该研究报告是关于克拉科的有丝分裂基因组的第一份报告，这将促进人们对C. fumosa和相关物种的遗传，进化和分类学的理解。