Non-invasive imaging of living cells is an advanced technique that is widely used in the life sciences and medical research. We demonstrate a refractive index quantification microscopy (RIQM) that enables label-free studies of glioma cell-substrate contacts involving cell adhesion molecules and the extracellular matrix. This microscopy takes advantage of the smallest available spot created when an azimuthally polarized perfect optical vortex beam (POV) is tightly focused with a first-order spiral phase, which results in a relatively high imaging resolution among biosensors. A high refractive index (RI) resolution enables the RI distribution within neuronal cells to be monitored. The microscopy shows excellent capability for recognizing cellular structures and activities, demonstrating great potential in biological sensing and live-cell kinetic imaging.

中文翻译：

---

使用折射率定量显微镜对细胞-基质接触进行高性能成像


活细胞无创成像是一种广泛应用于生命科学和医学研究的先进技术。我们展示了一种折射率定量显微镜（RIQM），可以对涉及细胞粘附分子和细胞外基质的神经胶质瘤细胞-基质接触进行无标记研究。该显微镜利用了方位偏振完美光学涡旋光束（POV）与一阶螺旋相位紧密聚焦时产生的最小可用光斑，从而在生物传感器中获得相对较高的成像分辨率。高折射率 (RI) 分辨率可以监测神经元细胞内的 RI 分布。该显微镜显示出出色的识别细胞结构和活动的能力，在生物传感和活细胞动力学成像方面展现出巨大的潜力。