To examine the cervical lesions by using the tumor-targeted near-infrared (NIR) fluorescent dyes TMTP1-PEG4-ICG, a dual modal colposcope with visible reflectance imaging and fluorescence imaging was developed. NIR fluorescence imaging and visible light reflectance imaging were integrated together on a colposcope by designing the specific optics and adopting a dual sensor charge coupled device (CCD) camera. Patients with squamous cell carcinoma, adenocarcinoma, cervical intraepithelial neoplasia (CIN) and cervicitis were examined using this dual modal colposcope to validate its potential of cervical cancer detection. Fluorescent dye TMTP1-PEG4-ICG was applied to the cervix 30 minutes before inspection. The fluorescence images were collected after wiping the unbound fluorescent dye using normal saline. Signal to back ratios (SBR) of the fluorescence images were analyzed and compared with the histological analysis. The results suggest that the fluorescent colposcope combined with tumor-specific near-infrared fluorescent dyes TMTP1-PEG4-ICG could help to evaluate cervical lesions in real time.

中文翻译：

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双模式荧光阴道镜结合近红外荧光染料TMTP1-PEG4-ICG检测宫颈病变

为了通过使用靶向肿瘤的近红外（NIR）荧光染料TMTP1-PEG4-ICG检查宫颈病变，开发了一种具有可见反射成像和荧光成像的双模式阴道镜。通过设计特定的光学器件并采用双传感器电荷耦合器件（CCD）相机，将NIR荧光成像和可见光反射成像集成到了阴道镜上。使用这种双模式阴道镜检查了鳞状细胞癌，腺癌，宫颈上皮内瘤样变（CIN）和宫颈炎患者，以验证其检测宫颈癌的潜力。在检查前30分钟将荧光染料TMTP1-PEG4-ICG涂在子宫颈上。使用生理盐水擦拭未结合的荧光染料后收集荧光图像。分析了荧光图像的信噪比（SBR），并与组织学分析进行了比较。结果表明，荧光阴道镜结合肿瘤特异性近红外荧光染料TMTP1-PEG4-ICG可以帮助实时评估宫颈病变。