Open-top light-sheet (OTLS) microscopes have been developed for user-friendly and versatile high-throughput 3D microscopy of thick specimens. As with all imaging modalities, spatial resolution trades off with imaging and analysis times. A hierarchical multi-scale imaging workflow would therefore be of value for many volumetric microscopy applications. We describe a compact multi-resolution OTLS microscope, enabled by a novel solid immersion meniscus lens (SIMlens), which allows users to rapidly transition between air-based objectives for low- and high-resolution 3D imaging. We demonstrate the utility of this system by showcasing an efficient 3D analysis workflow for a diagnostic pathology application.

中文翻译：

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多分辨率开放式顶片显微镜，可实现高效的3D病理学工作流程

开放式顶片（OTLS）显微镜已经开发出来，可用于厚样本的用户友好型多功能高通量3D显微镜检查。与所有成像方式一样，空间分辨率与成像和分析时间之间需要权衡。因此，分级的多尺度成像工作流程对于许多体积显微镜应用而言都是有价值的。我们描述了一种紧凑的多分辨率OTLS显微镜，该显微镜由新型固体浸没弯月形透镜（SIMlens）启用，它使用户可以在基于空气的物镜之间快速转换，以进行低分辨率和高分辨率3D成像。我们通过展示用于诊断病理学应用程序的高效3D分析工作流程来演示该系统的实用性。