We propose label-free and motion-free resolution-enhanced intensity diffraction tomography (reIDT) recovering the 3D complex refractive index distribution of an object. By combining an annular illumination strategy with a high numerical aperture (NA) condenser, we achieve near-diffraction-limited lateral resolution of 346 nm and axial resolution of 1.2 μm over 130 μm×130 μm×8 μm volume. Our annular pattern matches the system’s maximum NA to reduce the data requirement to 48 intensity frames. The reIDT system is directly built on a standard commercial microscope with a simple LED array source and condenser lens adds-on, and promises broad applications for natural biological imaging with minimal hardware modifications. To test the capabilities of our technique, we present the 3D complex refractive index reconstructions on an absorptive USAF resolution target and Henrietta Lacks (HeLa) and HT29 human cancer cells. Our work provides an important step in intensity-based diffraction tomography toward high-resolution imaging applications.

中文翻译：

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高数值孔径无标记显微镜中的分辨率增强强度衍射断层扫描

我们提出了无标记和无运动分辨率增强型衍射断层扫描 (reIDT)，可恢复物体的 3D 复折射率分布。通过将环形照明策略与高数值孔径 (NA) 聚光镜相结合，我们在 130 μm×130 μm×8 μm 的体积上实现了 346 nm 的近衍射极限横向分辨率和 1.2 μm 的轴向分辨率。我们的环形图案与系统的最大 NA 相匹配，以将数据要求减少到 48 个强度帧。reIDT 系统直接建立在带有简单 LED 阵列光源和聚光镜附加组件的标准商用显微镜上，并有望通过最少的硬件修改为自然生物成像提供广泛的应用。为了测试我们技术的能力，我们在吸收性美国空军分辨率目标和 Henrietta Lacks (HeLa) 和 HT29 人类癌细胞上展示了 3D 复折射率重建。我们的工作为基于强度的衍射断层扫描向高分辨率成像应用迈出了重要的一步。