Oligo-DNA Stoichiometrically Binds β-1,3-Glucan with the Best Fit Length,Biomacromolecules

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Oligo-DNA Stoichiometrically Binds β-1,3-Glucan with the Best Fit Length
Biomacromolecules ( IF 5.5 ) Pub Date : 2020-11-13 , DOI: 10.1021/acs.biomac.0c01038
Kazuki Sumiya ₁ , Takuya Matsunaga ₁ , Motoko Tanaka ₁ , Shinichi Mochizuki ₁ , Kazuo Sakurai ₁

Affiliation

Oligo-deoxyadenylic acid (dA_X) forms a novel 1:2 triple-helix with β-1,3-d-glucan schizophyllan (SPG). We found that dA_X meticulously selects the most suitable length of SPG to bind; for example, dA₃₀ only complexes with a short SPG chain having 30, 60, or 90 main-chain glucoses, and they can be easily isolated with each other. This study demonstrated such a novel stoichiometric complex formation by using gel permeation chromatography coupled with multi-angle light scattering and synchrotron small-angle X-ray scattering. These oligo-DNA/polysaccharide complexes can be used as a tool for delivering therapeutic oligonucleotides to immunocytes that express the β-1,3-d-glucan receptors. The present study provides a robust platform technique to characterize them in terms of modern regulatory science of nanomedicines, which is requisite to transfer drug candidates into clinical trial. Our findings are important for characterizing these complexes as well as for providing a new insight into nucleotide and saccharide chemistry.

中文翻译：

化学计量学上寡核苷酸-DNA以最佳适合长度结合β-1,3-葡聚糖

寡聚脱氧腺苷酸（dA _X）与β-1,3- d-葡聚糖schizophyllan（SPG）形成新的1：2三螺旋。我们发现dA _X精心选择了最合适的SPG长度进行结合。例如，dA ₃₀仅与具有30、60或90个主链葡萄糖的SPG短链形成复合物，它们可以轻松地彼此分离。这项研究通过结合多角度光散射和同步加速器小角度X射线散射的凝胶渗透色谱法证明了这种新颖的化学计量配合物的形成。这些寡聚DNA /多糖复合物可用作将治疗性寡核苷酸递送至表达β-1,3- d的免疫细胞的工具-葡聚糖受体。本研究提供了一个强大的平台技术，可以根据现代纳米药物管理科学对它们进行表征，这是将候选药物转移到临床试验中所必需的。我们的发现对于表征这些复合物以及提供对核苷酸和糖化学的新见解非常重要。

更新日期：2020-12-14

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