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Milk fatty acid composition, rumen microbial population and animal performance in response to diets rich in linoleic acid supplemented with Piper betle leaves in Saanen goats
Animal Production Science ( IF 1.4 ) Pub Date : 2020-01-01 , DOI: 10.1071/an20182
R. A. P. Purba , C. Yuangklang , S. Paengkoum , P. Paengkoum

Context Rumen biohydrogenation is an important way to produce conjugated linoleic acid (CLA), especially the rumenic acid isomer. However, CLA is principally synthesised endogenously in lactating mammals by delta 9-desaturase in breast tissue. Aims The aim of the study was to evaluate milk fatty acid profile, rumen microbial population and animal performance in response to diets containing sunflower oil either supplemented with or without flavonoids and essential oils from Piper betle L. powder (PP) in dairy goats. Method Twelve multiparous Saanen goats (42 ± 1.00 kg; mean ± s.d.) were randomly assigned to two treatment groups in an experiment that lasted for 6 weeks. The two experimental diets formulated as total mixed ration were: control (CTH) diet (containing 0% PP) and DPB diet (CTH diet containing 1.3% PP on a dry-matter basis). Key results Inclusion of flavonoids and essential oils from PP in the diet (DPB) did not affect dry-matter intake but resulted in a greater milk yield and altered the composition of milk. Compared with the control diet (CTH), the DPB diet decreased the saturated fatty acid concentration and increased the unsaturated fatty acid concentration in milk. Inclusion of PP decreased the C18:0 production (P < 0.05), resulting in higher C18:1 trans11 and C18:2 cis9 trans11 (P < 0.05) concentrations. Overall, DPB diet increased the total CLA by 1.5-fold, from 1.77 to 2.62 g/100 g fatty acid. The desaturase rate (except desaturase for carbon 18, P < 0.05), and atherogenic and thrombogenic indices were not affected by inclusion of PP in the DPB diet. Moreover, the DPB diet escalated total volatile fatty acid production and altered the volatile fatty acid profile. Compared with goats fed with CTH diet, PP supplementation increased the presence of ruminal Butyrivibrio fibrisolvens by ~5-fold, but the presence of B. proteoclasticus decreased to about 1/11 of the control. Conclusions The use of sunflower oil at 17.6 g/kg diet and inclusion of a practical dose of flavonoids and essential oils from Piper betle L. leaves in the diet of dairy goats can be an efficient method to improve milk yield and milk composition, including increasing the CLA concentration of milk. Implications These results constitute an alternative strategy to improve milk quality, without negatively affecting animal performance.

中文翻译:

萨能山羊奶脂肪酸组成、瘤胃微生物种群和动物对富含亚油酸和蒺藜叶的饮食的反应

背景 瘤胃生物氢化是生产共轭亚油酸(CLA),尤其是瘤胃酸异构体的重要途径。然而,CLA 主要是通过乳腺组织中的 delta 9-去饱和酶在哺乳期哺乳动物中内源性合成的。目的 本研究的目的是评估奶山羊的乳脂肪酸谱、瘤胃微生物种群和动物对含有葵花籽油的日粮的反应,日粮中添加或不添加黄酮类化合物和来自 Piper betle L.粉 (PP) 的精油。方法 在一项持续 6 周的实验中,十二只经产的萨能山羊(42 ± 1.00 kg;平均值 ± sd)被随机分配到两个治疗组。配制为总混合日粮的两种实验日粮是:对照 (CTH) 日粮(含有 0% PP)和 DPB 日粮(CTH 日粮,以干物质为基础含有 1.3% PP)。主要结果 在日粮 (DPB) 中包含来自 PP 的黄酮类化合物和精油不会影响干物质摄入量,但会导致更高的产奶量并改变牛奶的成分。与对照日粮(CTH)相比,DPB日粮降低了牛奶中的饱和脂肪酸浓度,增加了不饱和脂肪酸的浓度。包含 PP 降低了 C18:0 的产生 (P < 0.05),导致更高的 C18:1 trans11 和 C18:2 cis9 trans11 (P < 0.05) 浓度。总体而言,DPB 饮食将总 CLA 增加了 1.5 倍,从 1.77 到 2.62 g/100 g 脂肪酸。去饱和酶速率(碳 18 去饱和酶除外,P < 0.05)以及致动脉粥样硬化和血栓形成指数不受在 DPB 饮食中加入 PP 的影响。而且,DPB 饮食增加了总挥发性脂肪酸的产生并改变了挥发性脂肪酸的分布。与饲喂 CTH 饲料的山羊相比,PP 补充剂使瘤胃溶纤溶丁弧菌的存在增加了约 5 倍,但 B. proteoclastus 的存在减少到对照的约 1/11。结论 使用 17.6 g/kg 日粮的葵花籽油,并在奶山羊日粮中加入实际剂量的蒺藜叶中的黄酮类化合物和精油,是提高产奶量和牛奶成分的有效方法,包括增加牛奶中的 CLA 浓度。意义 这些结果构成了一种提高牛奶质量的替代策略,同时不会对动物生产性能产生负面影响。PP 补充使瘤胃溶纤溶丁弧菌的存在增加了约 5 倍,但 B. proteoclastus 的存在减少到对照的约 1/11。结论 使用 17.6 g/kg 日粮的葵花籽油,并在奶山羊日粮中加入实际剂量的蒺藜叶中的黄酮类化合物和精油,是提高产奶量和牛奶成分的有效方法,包括增加牛奶中的 CLA 浓度。含义 这些结果构成了一种提高牛奶质量的替代策略,同时不会对动物生产性能产生负面影响。PP 补充使瘤胃溶纤溶丁弧菌的存在增加了约 5 倍,但 B. proteoclastus 的存在减少到对照的约 1/11。结论 使用 17.6 g/kg 日粮的葵花籽油,并在奶山羊日粮中加入实际剂量的蒺藜叶中的黄酮类化合物和精油,是提高产奶量和牛奶成分的有效方法,包括增加牛奶中的 CLA 浓度。意义 这些结果构成了一种提高牛奶质量的替代策略,同时不会对动物生产性能产生负面影响。奶山羊饮食中的叶子可以是提高牛奶产量和牛奶成分的有效方法,包括增加牛奶的 CLA 浓度。含义 这些结果构成了一种提高牛奶质量的替代策略,同时不会对动物生产性能产生负面影响。奶山羊饮食中的叶子可以是提高牛奶产量和牛奶成分的有效方法,包括增加牛奶的 CLA 浓度。意义 这些结果构成了一种提高牛奶质量的替代策略,同时不会对动物生产性能产生负面影响。
更新日期:2020-01-01
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