Vibrio parahaemolyticus is the most common cause of seafood-borne illness reported in the United States. The draft genomes of 132 North American clinical and oyster V. parahaemolyticus isolates were sequenced to investigate their phylogenetic and biogeographic relationships. The majority of oyster isolate sequence types (STs) were from a single harvest location; however, four were identified from multiple locations. There was population structure along the Gulf and Atlantic Coasts of North America, with what seemed to be a hub of genetic variability along the Gulf Coast, with some of the same STs occurring along the Atlantic Coast and one shared between the coastal waters of the Gulf and those of Washington State. Phylogenetic analyses found nine well-supported clades. Two clades were composed of isolates from both clinical and oyster sources. Four were composed of isolates entirely from clinical sources, and three were entirely from oyster sources. Each single-source clade consisted of one ST. Some human isolates lack tdh, trh, and some type III secretion system (T3SS) genes, which are established virulence genes of V. parahaemolyticus. Thus, these genes are not essential for pathogenicity. However, isolates in the monophyletic groups from clinical sources were enriched in several categories of genes compared to those from monophyletic groups of oyster isolates. These functional categories include cell signaling, transport, and metabolism. The identification of genes in these functional categories provides a basis for future in-depth pathogenicity investigations of V. parahaemolyticus.

中文翻译：

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从全基因组序列数据推断北美副溶血性弧菌菌株的系统发育和生物地理学模式


副溶血性弧菌是美国报告的海鲜传播疾病的最常见原因。对 132 个北美临床和牡蛎副溶血弧菌分离株的基因组草案进行了测序，以研究它们的系统发育和生物地理学关系。大多数牡蛎分离序列类型 (ST) 来自单一收获地点；然而，在多个地点发现了四人。北美墨西哥湾和大西洋沿岸存在种群结构，墨西哥湾沿岸似乎是遗传变异的中心，一些相同的 ST 发生在大西洋沿岸，另一种则在墨西哥湾沿岸水域之间共享。和华盛顿州的。系统发育分析发现了九个得到充分支持的进化枝。两个进化枝由来自临床和牡蛎来源的分离株组成。其中四种完全由临床来源的分离株组成，三种完全来自牡蛎来源。每个单源进化枝由一个 ST 组成。一些人类分离株缺乏tdh 、 trh和一些 III 型分泌系统 (T3SS) 基因，这些基因是副溶血性弧菌已确定的毒力基因。因此，这些基因对于致病性并不是必需的。然而，与牡蛎分离株单系群相比，来自临床来源的单系群中的分离株富含几类基因。这些功能类别包括细胞信号传导、运输和代谢。这些功能类别中基因的鉴定为未来副溶血性弧菌致病性的深入研究奠定了基础。