In this study, chicken peptone was produced by hydrolyzing inedible parts derived from chickens using endo-protease and exo-protease. The usefulness of chicken peptone as a nutrient source for bacteria was evaluated in comparison with other commercially produced peptones (animal, soy and casein-derived peptone). Escherichia coli and Bacillus subtilis were used as test strains to determine the effect of peptones from different sources on their growth ability. Both bacteria were successfully cultured in chicken peptone solution, which is similar to peptone solution containing commercial peptones apart from animal peptone. In chemical analysis, chicken peptone contained 12.0% nitrogen; this was similar to the nitrogen content from other commercial peptone sources, except for the 9.0% nitrogen found in soy peptones. Molecular weight of the peptone was determined by gel filtration chromatography, and those of all peptone, except animal-derived peptone, were found to be less than 5000 Da. In addition, When B. subtilis was cultured in a medium containing chicken peptone, it was shown that the protease activity was highest as compared with other commercial peptones. From these results, it is suggested that chicken peptone can be utilized for microbial culture, and this is an effective method to reuse chicken waste.

中文翻译：

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鸡粪蛋白胨作为微生物氮源的评价

在这项研究中，鸡蛋白胨是通过使用内切蛋白酶和外切蛋白酶水解鸡的不可食用部分来生产的。与其他商业生产的蛋白胨（动物、大豆和酪蛋白衍生的蛋白胨）相比，鸡蛋白胨作为细菌营养源的有效性进行了评估。大肠杆菌和枯草芽孢杆菌被用作测试菌株，以确定不同来源的蛋白胨对其生长能力的影响。这两种细菌都在鸡蛋白胨溶液中成功培养，鸡蛋白胨溶液类似于含有商业蛋白胨的蛋白胨溶液，除了动物蛋白胨。化学分析，鸡蛋白胨含氮12.0%；这与其他商业蛋白胨来源的氮含量相似，但大豆蛋白胨中的氮含量为 9.0%。通过凝胶过滤色谱法测定蛋白胨的分子量，发现除动物源蛋白胨外的所有蛋白胨均小于5000 Da。此外，当 B. subtilis 在含有鸡蛋白胨的培养基中培养时，与其他商业蛋白胨相比，蛋白酶活性最高。这些结果表明，鸡蛋白胨可用于微生物培养，这是一种有效的鸡粪再利用方法。