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Functional roles of the hexamer structure of C‐phycocyanin revealed by calculation of absorption wavelength
FEBS Open Bio ( IF 2.8 ) Pub Date : 2020-11-15 , DOI: 10.1002/2211-5463.13038
Hiroto Kikuchi 1
Affiliation  

Cyanophyta‐phycocyanin (C‐PC) is the main constituent of the rod of phycobilisome (PBS), which is a highly ordered and large peripheral light‐harvesting protein complex present on the cytoplasmic side of the thylakoid membrane in cyanobacteria and red algae. The C‐PC monomer comprises two chains, α‐ and β‐subunits, and aggregates to form ring‐shaped trimers (αβ)3 with rotational symmetry. The ring‐shaped trimer (αβ)3 is a structural block unit (SBU) that forms the rod of PBS. Two (αβ)3 SBUs are arranged in a face‐to‐face manner to form an (αβ)6‐hexamer. In this study, the electronic states of three phycocyanobilins, α84, β84, and β155 in C‐phycocyanin, constituting the rod of the PBS, were calculated for both the trimer and hexamer models by considering the effect of the electrostatic field of protein moieties and water molecules. For the hexamer, the absorption wavelengths of α84, β84, and β155 were similar to those obtained experimentally; however, for the trimer, only the absorption wavelength of β155 shifted toward a shorter‐wavelength. The nature of the hexamer structure as a hierarchical structure is revealed by considering the calculated absorption wavelength and energy transfer.

中文翻译:

通过吸收波长计算揭示C-藻蓝蛋白六聚体结构的功能作用

蓝藻藻蓝蛋白(C-PC)是藻胆体(PBS)杆的主要成分,藻胆体杆是一种高度有序的大型外周光捕获蛋白复合物,存在于蓝细菌和红藻的类囊体膜的细胞质侧。C-PC单体包含两条链、α-和β-亚基,并聚集形成具有旋转对称性的环状三聚体(αβ) 3 。环形三聚体 (αβ) 3是形成 PBS 杆的结构嵌段单元 (SBU)。两个(αβ) 3 SBU以面对面的方式排列形成(αβ) 6六聚体。在这项研究中,通过考虑蛋白质部分静电场的影响,计算了构成 PBS 棒的 C-藻蓝蛋白中三种藻蓝蛋白 α84、β84 和 β155 的电子态,计算了三聚体和六聚体模型。水分子。对于六聚体,α84、β84和β155的吸收波长与实验获得的吸收波长相似;然而,对于三聚体,只有β155的吸收波长向更短的波长移动。通过考虑计算的吸收波长和能量转移,揭示了六聚体结构作为分层结构的本质。
更新日期:2021-01-04
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