Rhopalosiphum padi is a worldwide agricultural pest. Chemosensory proteins (CSPs) are considered to be a type of transporters which can bind chemicals from external environments. Previous research showed that the expression of some insect CSPs were significantly increased after exposure to insecticides, and CSPs were involved in insecticide resistance or susceptibility. However, the role of CSPs in the susceptibility and response of R. padi to insecticides is still unknown. In this study, we identified eight CSP (RpCSP) from R. padi by genome‐wide investigation. Seven RpCSP genes had two exons, while RpCSP7 had three exons. qPCR analyses showed that the mRNA levels of the eight RpCSP genes were significantly affected by imidacloprid and beta‐cypermethrin in different post‐treatment periods. Molecular docking predicted that there were hydrogen bonding sites which played key roles in binding of RpCSP4, RpCSP5, RpCSP6, RpCSP7 and RpCSP10 with imidacloprid and beta‐cypermethrin. Knockdown of RpCSP4, RpCSP5, RpCSP6 and RpCSP10 by RNA interference significantly increased the aphid mortality under two sublethal concentrations of imidacloprid. Mortalities under two sublethal concentrations of beta‐cypermethrin conditions were significantly higher after injection of R. padi with dsCSP4 and dsCSP6. The results indicate that some RpCSP genes are involved in the insecticide susceptibility of R. padi.

中文翻译：

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化学感应蛋白参与小麦作物的严重害虫 Rhopalosiphum padi 的杀虫剂敏感性

Rhopalosiphum padi是一种世界性的农业害虫。化学感应蛋白 (CSP) 被认为是一种转运蛋白，可以结合来自外部环境的化学物质。以往的研究表明，一些昆虫CSPs在接触杀虫剂后表达显着增加，CSPs与杀虫剂抗性或敏感性有关。然而，CSP 在R. padi对杀虫剂的敏感性和反应中的作用仍然未知。在这项研究中，我们通过全基因组调查从R. padi 中鉴定了 8 个CSP ( RpCSP ) 。七个RpCSP基因有两个外显子，而RpCSP7有三个外显子。qPCR 分析表明，8 个RpCSP基因的 mRNA 水平在不同的治疗后时期受到吡虫啉和 β-氯氰菊酯的显着影响。分子对接预测存在氢键位点，在RpCSP4、RpCSP5、RpCSP6、RpCSP7和RpCSP10与吡虫啉和 β-氯氰菊酯的结合中起关键作用。敲除 RpCSP4、RpCSP5、RpCSP6和RpCSP10在两种亚致死浓度的吡虫啉下，RNA 干扰显着增加了蚜虫的死亡率。用 ds CSP4和 ds CSP6注射R. padi后，两种亚致死浓度的 β-氯氰菊酯条件下的死亡率显着更高。结果表明，一些RpCSP基因参与的药剂敏感性R.蚜。