Human epidermal growth factor (hEGF) plays an important role in the growth and division of epithelial cells and has good application prospects in skin-related injuries and diseases. Weak skin penetration and rapid clearance of hEGF in skin via the mononuclear phagocyte system have restricted the application of hEGF. To overcome these shortcomings, the recombinant gene TAT-hEGF-CD47 was constructed in our experiments, and the fusion protein TAT-hEGF-CD47 was expressed, purified and renatured. The cell proliferation-promoting function, skin penetration and concentration of TAT-hEGF-CD47 in skin after its application were determined. The results showed that TAT-hEGF-CD47 effectively promoted human skin fibroblast and skin epithelial cell proliferation, and the proliferation-promoting effect was positively correlated with the TAT-hEGF-CD47 concentration. After administration to the skin, TAT-hEGF-CD47 effectively penetrated the epidermal layer of the skin because of the TAT domain and stayed in the skin for a long time because the CD47 fragment slowed its clearance via the mononuclear phagocytic system. In conclusion, TAT-hEGF-CD47 exhibits high cell proliferation-promoting activity, high skin penetration efficiency and long retention time in skin and has laid the foundation for its wide application in skin repair, ulcer, diabetes and even cancer treatments.

人表皮生长因子（hEGF）在上皮细胞的生长和分裂中起重要作用，在皮肤相关损伤和疾病中具有良好的应用前景。皮肤渗透力较弱，hEGF 通过单核吞噬细胞系统在皮肤中的快速清除限制了 hEGF 的应用。为了克服这些缺点，我们在实验中构建了重组基因TAT-hEGF-CD47，并对融合蛋白TAT-hEGF-CD47进行了表达、纯化和复性。测定其应用后TAT-hEGF-CD47的细胞增殖促进功能、皮肤渗透和皮肤中的浓度。结果显示TAT-hEGF-CD47有效促进人皮肤成纤维细胞和皮肤上皮细胞增殖，促增殖作用与TAT-hEGF-CD47浓度呈正相关。给予皮肤后，TAT-hEGF-CD47由于TAT结构域有效穿透皮肤表皮层，并在皮肤中停留很长时间，因为CD47片段通过单核吞噬系统减慢其清除速度。综上所述，TAT-hEGF-CD47具有高促细胞增殖活性、高皮肤渗透效率和长在皮肤中的停留时间，为其在皮肤修复、溃疡、糖尿病甚至癌症治疗中的广泛应用奠定了基础。TAT-hEGF-CD47由于存在TAT结构域而有效穿透皮肤表皮层并在皮肤中停留很长时间，因为CD47片段通过单核吞噬系统减慢了其清除速度。综上所述，TAT-hEGF-CD47具有高促细胞增殖活性、高皮肤渗透效率和长在皮肤中的停留时间，为其在皮肤修复、溃疡、糖尿病甚至癌症治疗中的广泛应用奠定了基础。TAT-hEGF-CD47由于存在TAT结构域而有效穿透皮肤表皮层并在皮肤中停留很长时间，因为CD47片段通过单核吞噬系统减慢了其清除速度。综上所述，TAT-hEGF-CD47具有高促细胞增殖活性、高皮肤渗透效率和长在皮肤中的停留时间，为其在皮肤修复、溃疡、糖尿病甚至癌症治疗中的广泛应用奠定了基础。