Wound dressings are typically used to provide a favorable environment supporting the intricate process of wound healing. This research aims to fabricate and evaluate an electrospun polycaprolactone (EsPCL) membrane coated with various densities of chitosan oligomers (COS) – a biological agent - for application as bioactive wound dressing. Weight calculation was employed to investigate the density of COS coated onto the electrospun PCL membrane. Physicochemical characteristics of the prepared membranes, such as hydrophilicity and mechanical properties were demonstrated and evaluated through standard experimental methods. In vitro assays and mice model were used to investigate the antibacterial activities, cytocompatibility, hemostasis and the in vivo interaction of the membranes. The results showed that COS was coated successfully on the surface of the polymeric membrane, altering its morphology and associated characteristics. The greater concentration of COS led to an increase in the thickness of the membrane, which resulted in stronger antibacterial activities. Moreover, the increase of chitosan oligomers density in the membrane induced faster hemostasis and affected the re-epithelialization and wound healing in mice. Thus, the membrane as a whole and particularly chitosan oligomers were shown to be potential for further studies regarding wound dressing.

伤口敷料通常用于提供有利的环境，以支持复杂的伤口愈合过程。这项研究旨在制造和评估涂有各种密度的壳聚糖低聚物（COS）（一种生物制剂）的电纺聚己内酯（EsPCL）膜，以用作生物活性伤口敷料。使用重量计算来研究涂覆到电纺PCL膜上的COS的密度。通过标准实验方法证明并评估了制备的膜的理化特性，例如亲水性和机械性能。使用体外试验和小鼠模型研究其抗菌活性，细胞相容性，止血和体内活性膜的相互作用。结果表明，COS成功地涂覆在聚合物膜的表面上，改变了它的形态和相关特性。较高的COS浓度导致膜厚度增加，从而导致更强的抗菌活性。此外，膜中壳聚糖低聚物密度的增加引起更快的止血，并影响了小鼠的上皮再形成和伤口愈合。因此，膜整体，特别是壳聚糖低聚物显示出对伤口敷料的进一步研究的潜力。