Abstract Polylactic acid-based nanoparticles are promising materials due to their tunable properties that are connected to surface modification. We have prepared polylactic acid nanospheres (PLA) with a non-magnetic phase (PLA loaded with various bovine serum albumin (BSA) concentrations) and magnetic phase (PLA loaded with magnetite nanoparticles coated with different amount of BSA (MFBSA)). The morphology, physico-chemical, magnetic and anti-amyloid properties were characterized by several biophysical methods. The amount of BSA used for PLA nanospheres or MFBSA modification played an important role in controlling the hydrodynamic size and zeta potential. In addition, magnetite loaded PLA nanospheres and MFBSA were all superparamagnetic. The potential of modified PLA nanospheres compared to MFBSA and 'naked' PLA to inhibit insulin amyloid fibrillation and destroy mature insulin fibrils was studied in vitro for samples with BSA/Fe3O4 weight ratio of 0.5. Destroying potential of samples was size–dependent, with the smallest MFBSA0.5 having the most significant effect. The strongest inhibitory effect on insulin fibrillation showed PLA-MFBSA0.5, slightly smaller than BSA modified PLA nanospheres.

中文翻译：

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改性聚合物纳米球——表征及其对胰岛素淀粉样蛋白聚集的抗淀粉样蛋白活性

摘要 聚乳酸基纳米粒子因其与表面改性相关的可调特性而成为有前景的材料。我们已经制备了具有非磁性相（PLA 载有各种牛血清白蛋白 (BSA) 浓度）和磁性相（PLA 载有磁铁矿纳米颗粒，涂有不同量的 BSA (MFBSA)）的聚乳酸纳米球 (PLA)。通过几种生物物理方法表征了形态学、物理化学、磁性和抗淀粉样蛋白特性。用于 PLA 纳米球或 MFBSA 改性的 BSA 量在控制流体动力学尺寸和 zeta 电位方面起着重要作用。此外，磁铁矿负载的 PLA 纳米球和 MFBSA 都是超顺磁性的。与 MFBSA 和“裸”相比，改性 PLA 纳米球的潜力 针对 BSA/Fe3O4 重量比为 0.5 的样品，在体外研究了 PLA 抑制胰岛素淀粉样蛋白纤维化和破坏成熟胰岛素原纤维。样品的破坏潜力取决于大小，最小的 MFBSA0.5 具有最显着的影响。对胰岛素纤颤的最强抑制作用显示 PLA-MFBSA0.5，略小于 BSA 修饰的 PLA 纳米球。