In Brazil, there have been few leptospires isolated from cattle, especially in the Amazon, implying that the epidemiology of the disease in this region is still largely unclear. In a previous study, 52 Leptospira isolates were obtained from urine of cattle raised in the Brazilian Amazon and, to achieve a greater understanding of Leptospira infection in cattle of this region, the present study aimed to serologically and molecularly characterizes all these isolates. The laboratory assays used were the microscopic agglutination test (MAT) adopting a panel of polyclonal antisera against Leptospira spp. for serogrouping the isolates, DNA sequencing (secY) and multiple locus variable number tandem repeat analysis (MLVA). The isolates belonged to five species: 20/52 were identified as L. borgpetersenii (38.5 %); 18/52 as L. kirschneri (34.6 %); 9/52 as L. santarosai (17.3 %); 3/52 as L. noguchii (5.8 %) and 2/52 as L. interrogans (3.8 %). With serogrouping, nine different serogroups were detected, with a high frequency of the Sejroe serogroup. MLVA showed that all L. borgpetersenii isolates had a profile compatible with serovar Hardjo; moreover, the other isolates demonstrated a diversity of patterns, and some of them may represent strains not yet characterized. In the Brazilian Amazon, the leptospires circulating in cattle revealed the unique aspects of infections in this area which, in addition to a variety of strains, were characterized by a high frequency of the Sejroe serogroup, highlighting the serovar Hardjo, which has not been reported in other regions of Brazil.

在巴西，很少有从牛身上分离出的钩端螺旋体，特别是在亚马逊河中，这表明该地区的流行病学仍不清楚。在先前的研究中，从巴西亚马逊河地区饲养的牛的尿液中获得了52株钩端螺旋体分离株，为进一步了解该地区牛的钩端螺旋体感染，本研究旨在对所有这些分离株进行血清学和分子学表征。所使用的实验室测定是采用一组针对钩端螺旋体的多克隆抗血清的显微凝集试验（MAT）。对分离物进行血清分组，DNA测序（secY）和多个基因座可变数串联重复分析（MLVA）。分离物属于五个物种：20/52被鉴定为博格特氏乳杆菌（38.5％）；五十二分之十八为L. kirschneri（34.6％）; 9/52为圣罗莎乳（17.3％）; 3/52为野口乳杆菌（5.8％）和2/52为询问乳杆菌（3.8％）。通过血清分组，检测到九种不同的血清群，其中Sejroe血清群的频率很高。MLVA显示所有的博格特氏菌分离株具有与血清型Hardjo相容的特性。此外，其他分离株表现出多种模式，其中一些可能代表尚未鉴定的菌株。在巴西的亚马逊河中，在牲畜中传播的钩端螺旋体揭示了该区域感染的独特方面，除了多种毒株之外，其特征还在于Sejroe血清群的高频率，突出了尚未报道的Hardjo血清群。在巴西其他地区。