The West Indian fruit fly, Anastrepha obliqua (Macquart), infests a wide diversity of tropical fruit. Previous studies suggest that A. obliqua adults are attracted to volatile compounds common in different hosts. However, to date, most studies have used ripe fruit for the identification of attractive compounds. In this study, we investigated the attraction of sexually mature A. obliqua females and males to two cultivars and three ripening stages of guava. We also identified the attractive compounds to A. obliqua by combined gas chromatography-electroantennographic detector (GC-EAD) and gas chromatography-mass spectrometry (GC–MS) and evaluated the biological activity of the identified compounds in field-cage tests. We found that individuals of both sexes of A. obliqua showed no preference to the volatiles of either of the two cultivars of guava evaluated. In contrast, flies were more attracted to ripe and half-ripe fruit than to unripe ones. GC-EAD analyses of extracts of ripe “Creole” or “Thai” cultivars identified six compounds that elicited antennal responses by A. obliqua females and males. The compounds were identified by GC–MS as ethyl butyrate, cis-3-hexen-1-ol, ethyl hexanoate, cis-3-hexenyl acetate, ethyl benzoate, and ethyl octanoate. Half-ripe guava emit ethyl butyrate, cis-3-hexen-1-ol, ethyl hexanoate, and cis-3-hexenyl acetate, while only traces of cis-3-hexenyl-acetate were found in unripe guava. Field-cage tests with synthetic standards confirmed that the compounds identified are responsible for the attraction of A. obliqua flies to ripe guava.

西印度果蝇Anastrepha obliqua（Macquart）感染了多种热带水果。以往的研究表明，一个。斜足成虫被不同宿主中常见的挥发性化合物所吸引。但是，迄今为止，大多数研究都使用成熟的果实来鉴定有吸引力的化合物。在这项研究中，我们调查了性成熟A的吸引力。蠖女性和男性对两个品种，番石榴三个成熟阶段。我们还确定了对A有吸引力的化合物。斜方肌通过气相色谱-电血管造影检测器（GC-EAD）和气相色谱-质谱（GC-MS）的组合，在现场笼测试中评估了所鉴定化合物的生物活性。我们发现A的两个性别的人。obliqua对所评估的两个番石榴品种中的任何一个的挥发物均无偏见。相反，成熟和半成熟的水果比未成熟的水果更吸引果蝇。GC-EAD对成熟的“克里奥尔”或“泰国”品种提取物的分析确定了6种化合物，它们通过A引起触角反应。斜方肌女性和男性。通过GC-MS鉴定出的化合物为丁酸乙酯，顺式-3-己烯-1-醇，己酸乙酯，顺式-3-己烯基乙酸酯，苯甲酸乙酯和辛酸乙酯。半成熟的番石榴散发出丁酸乙酯，顺式-3-己烯-1-醇，己酸乙酯和顺式-3-己烯基乙酸酯，而未熟的番石榴中仅发现痕量的顺式-3-己烯基乙酸酯。用合成标准品进行的笼养试验证实，所鉴定的化合物是A吸引的原因。li蝇飞到成熟的番石榴。