Purpose

To evaluate the prevalence of Cryptosporidium and Microsporidia, associated risk factors and species identification in patients with haematological malignancies (HM).

Methods

A total of 148 consecutive patients with HM and 101 healthy subjects were evaluated for Cryptosporidium and Microsporidia using modified Kinyoun and modified Trichrome staining. Clinical, demographic and laboratory parameters were studied. The species of Cryptosporidium and Microsporidia were studied using PCR–RFLP.

Results

Of 148 HM patients initially screened, 47 were excluded from the final analysis due to inadequate clinical records. Patients with HM [n = 101, 63 (62.4%) male] more often had Cryptosporidium than healthy subjects [n = 101, 65 (74.4%) male] [3/101 (3%) vs. 0/101 (0%), p = 0.02]. Two of 101 (2%) HM patients and none of the healthy subjects had Microsporidia (p = 0.155). Diarrhea was more prevalent in HM patients with Cryptosporidium than those without [3, 100% vs. 39/96, 40.62%; p = 0.04). Both patients infected with Microsporidia presented with persistent diarrhea and fever. Cryptosporidium hominis was identified in all the three HM patients. Enterocytozoon bieneusi was identified in one HM patient infected with Microsporidia, which was classified as genotype Ind2.

Conclusion

Cryptosporidium and Microsporidia may infect HM patients leading to overwhelming diarrhea. The commonest species of Cryptosporidium and Microsporidia found to infect HM patients are C. hominis and E. bieneusi.

中文翻译：

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感染血液系统恶性肿瘤患者的隐孢子虫和微孢子虫的患病率和遗传特征

目的

评估血液系统恶性肿瘤 (HM) 患者中隐孢子虫和微孢子虫的流行、相关危险因素和物种鉴定。

方法

使用改良的 Kinyoun 和改良的三色染色对总共 148 名连续 HM 患者和 101 名健康受试者进行了隐孢子虫和微孢子虫评估。研究了临床、人口统计学和实验室参数。使用 PCR-RFLP 研究了隐孢子虫和微孢子虫的种类。

结果

在最初筛选的 148 名 HM 患者中，47 名因临床记录不充分而被排除在最终分析之外。患者HM [N = 101，63（62.4％）男性]更经常不得不隐比对0/101（0％健康受试者[N = 101，65（74.4％）男性] [3/101（3％） ), p  = 0.02]。101 名 (2%) HM 患者中有两名 (2%) 和健康受试者均未患有微孢子虫 ( p  = 0.155)。患有隐孢子虫的HM 患者的腹泻比没有隐孢子虫的患者更普遍[3, 100% vs. 39/96, 40.62%; p  = 0.04）。两名感染微孢子虫的患者均出现持续腹泻和发烧。在所有三名 HM 患者中都发现了人隐孢子虫。双肠细胞虫 在一名感染了微孢子虫的 HM 患者中发现了这种病毒，该微孢子虫被归类为基因型 Ind2。

结论

隐孢子虫和微孢子虫可能感染 HM 患者，导致严重腹泻。发现感染 HM 患者的最常见的隐孢子虫和微孢子虫物种是C. hominis和E. bieneusi。