The present study investigated the association between single nucleotide polymorphisms (SNPs) in leptin gene with various economic traits (body weight at different age, first lactation reproduction and production traits) in Sahiwal cows. In order to detect genetic variants in intron 2 (422 bp), exon 2 (94 bp) and two regions of exon 3 (331 and 317 bp) of leptin, polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) method was used and revealed two transitions at positions T93262901C of intron 2 and C93263979T of exon 3. General linear model (GLM) analysis revealed significant association of T93262901C SNP with weight at first service (WFS), weight at first calving (WFC), first service period (FSP), first calving interval (FCI) and FL305DMY while SNP C93263979T was found to be significantly associated with birth weight (wt), 30 months wt, AFS and AFC. The investigation indicated that heterozygous genotype had a trend for better production with optimum growth and reproduction as compared to homozygote. The present study supports that SNP in leptin gene can be used as an aid to selection for improving different economic traits in Sahiwal cows.

中文翻译：

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萨希瓦尔奶牛瘦素基因多态性与生长，育性和产奶性状的关系

本研究调查了瘦肉牛基因瘦素基因中的单核苷酸多态性（SNP）与各种经济性状（不同年龄的体重，首次泌乳繁殖和生产性状）之间的关系。为了检测瘦蛋白的内含子2（422 bp），外显子2（94 bp）和外显子3的两个区域（331和317 bp）的遗传变异，采用聚合酶链反应-限制性片段长度多态性（PCR-RFLP）方法使用并揭示了内含子2的位置T93262901C和外显子3的C93263979T的两个过渡。一般线性模型（GLM）分析显示，T93262901C SNP与首次使用重量（WFS），第一次产犊（WFC），第一次使用时间的体重之间存在显着关联（FSP），第一次产犊间隔（FCI）和FL305DMY，而发现SNP C93263979T与出生体重（wt）显着相关，30个月wt，AFS和AFC。研究表明，与纯合子相比，杂合基因型具有更好的生产，最佳的生长和繁殖趋势。本研究支持瘦素基因中的SNP可以用作选择以改善Sahiwal奶牛不同经济性状的辅助手段。