Iron-meditated fungal starvation by lupine rhizosphere-associated and extremotolerant Streptomyces sp. S29 desferrioxamine production,Molecular Omics

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Iron-meditated fungal starvation by lupine rhizosphere-associated and extremotolerant Streptomyces sp. S29 desferrioxamine production
Molecular Omics ( IF 3.0 ) Pub Date : 2020-11-9 , DOI: 10.1039/d0mo00084a
Scott A Jarmusch ₁ , Diego Lagos-Susaeta , Emtinan Diab , Oriana Salazar , Juan A Asenjo , Rainer Ebel , Marcel Jaspars

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Siderophores are iron-chelating compounds that aid iron uptake, one of the key strategies for microorganisms to carve out ecological niches in microbially diverse environments. Desferrioxamines are the principal siderophores produced by Streptomyces spp. Their biosynthesis has been well studied and as a consequence, the chemical potential of the pathway continues to expand. With all of this in mind, our study aimed to explore extremotolerant and lupine rhizosphere-derived Streptomyces sp. S29 for its potential antifungal capabilities. Cocultivation of isolate S29 was carried out with Aspergillus niger and Botrytis cinerea, both costly fungal phytopathogens in the wine industry, to simulate their interaction within the rhizosphere. The results indicate that not only is Streptomyces sp. S29 extraordinary at producing hydroxamate siderophores but uses siderophore production as a means to ‘starve’ the fungi of iron. High resolution LC-MS/MS followed by GNPS molecular networking was used to observe the datasets for desferrioxamines and guided structure elucidation of new desferrioxamine analogues. Comparing the new chemistry, using tools like molecular networking and MS2LDA, with the known biosynthesis, we show that the chemical potential of the desferrioxamine pathway has further room for exploration.

中文翻译：

羽扇豆根际相关和极端耐受的链霉菌属对铁介导的真菌饥饿。S29 去铁胺生产

铁载体是帮助铁吸收的铁螯合化合物，是微生物在微生物多样化环境中开辟生态位的关键策略之一。去铁胺是链霉菌属产生的主要铁载体。它们的生物合成已得到充分研究，因此该途径的化学潜力继续扩大。考虑到所有这些，我们的研究旨在探索极端耐受和羽扇豆根际衍生的链霉菌属。S29 具有潜在的抗真菌能力。分离株 S29 与黑曲霉和灰葡萄孢的共培养，这两种是葡萄酒行业中昂贵的真菌植物病原体，以模拟它们在根际内的相互作用。结果表明，不仅链霉菌属。S29 在生产异羟肟酸铁载体方面非常出色，但使用铁载体生产作为“饿死”真菌铁的一种手段。高分辨率 LC-MS/MS 和 GNPS 分子网络用于观察去铁胺的数据集和新去铁胺类似物的引导结构阐明。使用分子网络和 MS2LDA 等工具将新化学与已知的生物合成进行比较，我们表明去铁胺途径的化学潜力有进一步的探索空间。

更新日期：2020-12-09

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